Statistical organelle dissection of Arabidopsis guard cells using image database LIPS.

Scientific Reports
Takumi HigakiSeiichiro Hasezawa

Abstract

To comprehensively grasp cell biological events in plant stomatal movement, we have captured microscopic images of guard cells with various organelles markers. The 28,530 serial optical sections of 930 pairs of Arabidopsis guard cells have been released as a new image database, named Live Images of Plant Stomata (LIPS). We visualized the average organellar distributions in guard cells using probabilistic mapping and image clustering techniques. The results indicated that actin microfilaments and endoplasmic reticulum (ER) are mainly localized to the dorsal side and connection regions of guard cells. Subtractive images of open and closed stomata showed distribution changes in intracellular structures, including the ER, during stomatal movement. Time-lapse imaging showed that similar ER distribution changes occurred during stomatal opening induced by light irradiation or femtosecond laser shots on neighboring epidermal cells, indicating that our image analysis approach has identified a novel ER relocation in stomatal opening.

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Citations

Jul 22, 2014·The New Phytologist·Deirdre H McLachlanSilke Robatzek
May 5, 2016·Journal of Microscopy·Mark D FrickerMichael J Deeks
Apr 29, 2016·The Plant Journal : for Cell and Molecular Biology·Eric BiotPhilippe Andrey
Jan 16, 2015·Scientific Reports·Takumi HigakiSeiichiro Hasezawa
Oct 29, 2013·Plant & Cell Physiology·Takumi HigakiSeiichiro Hasezawa
May 10, 2016·Journal of Biomedical Optics·Atsuhiro IshiiMitsuhiro Terakawa
Feb 28, 2020·Journal of Cell Science·Liam ElliottCharlotte Kirchhelle

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Methods Mentioned

BETA
transgenic

Software Mentioned

ImageJ
KbiStkFilter
KbiClustering
JAVA
Abobe Photoshop

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