Status epilepticus alters hippocampal PKAbeta and PKAgamma expression in mice

Seizure : the Journal of the British Epilepsy Association
Jian Xin LiuFeng Ru Tang

Abstract

To investigate the localization and progressive changes of cyclic-AMP dependent protein kinase (cPKA) in the mouse hippocampus at acute stages during and after pilocarpine induced status epilepticus. Pilocarpine induced status epilepticus mice were sacrificed 30 min, 2 h or 1 day after the start of a approximately 7 h lasting status as assessed by video-electroencephalography. Brains were processed for quantitative immunohistochemistry of hippocampal cPKAbeta and cPKAgamma, and immunohistochemical co-localization of cPKAbeta and cPKAgamma with calbindin (CB), calretinin (CR), and parvalbumin (PV). Based on anatomical and morphological assessment, cPKAbeta was primarily expressed by principal cells and cPKAgamma by interneurons. In CA1, cPKAbeta co-localized with 76% of CB, 41% of CR, and 95% of PV-immunopositive cells, while cPKAgamma co-localized with 50% of CB, 29% of CR, and 80% of PV-immunopositive cells. Upon induction of status epilepticus, cPKAbeta expression was transiently reduced in CA1, whereas cPKAgamma expression was sustainably reduced. cPKA may play an important role in neuronal hyperexcitability, death and epileptogenesis during and after pilocarpine induced status epilepticus.

References

Jan 27, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amanda M VanhooseDanny G Winder
Feb 22, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Veronika Zsiros, Gianmaria Maccaferri
Jul 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Boris D HeifetsPablo E Castillo
Aug 8, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sanjay N RakhadeFrances E Jensen

❮ Previous
Next ❯

Citations

Jul 30, 2014·Biochemical Pharmacology·Lisa AdelfingerBernhard Bettler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.