PMID: 9420869Jul 1, 1995Paper

Status of hCG/LH receptor and G proteins in human endometrium during artificial cycles of hormone replacement therapy

Journal of the Society for Gynecologic Investigation
L BernardiniJ P Balmaceda

Abstract

We examined the existence of hCG/LH receptors and associated GTP-binding (G) proteins in membrane fractions of nonpregnant human endometrium and investigated whether their expression is affected, in vivo, by estrogen and progesterone replacement therapy. A pool of normal endometrial biopsy specimens (n = 5) was initially used to characterize receptors and G proteins. Subsequently, biopsy specimens (n = 22) were obtained from 11 patients undergoing evaluation cycles of hormone replacement therapy (HRT). From each patient, two specimens were collected on successive cycle days: on day 0 (last day of estrogen) and on either day 3, 6, or 9 of progesterone supplementation. Both hCG/LH receptor and G proteins were determined in membrane (10,000 x g) fractions by immunoblot analysis using specific polyclonal antibodies against synthetic fragments of hCG/LH receptor and against G proteins. Membrane fractions from rat brain and rat corpus luteum were used as controls. Proteins were loaded on the gel under reducing conditions. The receptor antibody immunoreacted with a protein of approximately 68 kd in endometrial membranes. A similar protein was detected in rat corpus luteum. The G-protein antibodies detected Gs alpha, Gi3 alpha, Gi1 alp...Continue Reading

Citations

Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·A T FazleabasJ B Miller
Nov 29, 2011·Reproductive Biomedicine Online·Delphine HaouziSamir Hamamah
Apr 5, 2003·Reproductive Biomedicine Online·Andreas HerrlerHenning M Beier
May 20, 2005·Reproductive Biomedicine Online·Serena EmilianiYvon Englert
Mar 21, 2007·Molecular and Cellular Endocrinology·P LichtL Wildt

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