Status of p16(INK4a) and E-cadherin gene promoter methylation in Moroccan patients with cervical carcinoma

Oncology Research
Mohammed AttalebMohammed El Mzibri

Abstract

Aberrant methylation of tumor suppressor gene promoters has been extensively investigated in cervical cancer. Transcriptional silencing, as a main consequence of hypermethylation of CpG islands, is the predominant mechanism of p16(INK4a) and E-cadherin gene inactivation in malignant epithelial tumors. This study was conducted to evaluate the promoter methylation status of p16(INK4a) and E-cadherin genes in 22 specimens of cervical carcinomas, four cervical cancer cell lines (HeLa, SiHa, Caski, C33A), and 20 human papillomavirus negative specimens, obtained from normal cervical swabs, using the methylation-specific PCR approach. Hypermethylation of the 5' CpG island of the p16(INK4a) and E-cadherin genes were found in 13 (59.1%) and 10 (45.5%) of 22 cervical cancer samples, respectively. Furthermore, our findings did not show any correlation between promoter methylation of p16(INK4a) and E-cadherin genes and clinicopathological parameters, including HPV infection, phenotypic distribution, and stage of the disease. However, hypermethylation of E-cadherin gene promoter appears to be age related in cervical cancer, whereas the frequency of aberrant methylation of p16(INK4a) gene promoter is unchanged according to the age of patient...Continue Reading

Citations

Sep 6, 2012·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S D SilvaL P Kowalski
Apr 21, 2017·The FEBS Journal·Boqian ZhuChengchun Tang
Jul 12, 2012·Oncology Letters·Abhimanyu Kumar JhaJagdeep Kaur
Oct 6, 2020·Indian Journal of Clinical Biochemistry : IJCB·M BanerjeeT K Saha

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