Oct 28, 2019

Staufen 1 amplifies pro-apoptotic activation of the unfolded protein response.

BioRxiv : the Preprint Server for Biology
Mandi GandelmanStefan M Pulst

Abstract

Staufen-1 (STAU1) is an RNA binding protein that becomes highly overabundant in numerous neurodegenerative disease models, including those carrying mutations in presenilin1 (PSEN1), microtubule associated protein tau (MAPT), huntingtin (HTT), TAR DNA-binding protein-43 gene (TARDBP) or C9orf72. We previously reported that elevations in STAU1 determine autophagy defects. Additional functional consequences of STAU1 overabundance, however, have not been investigated. We studied the role of STAU1 in the chronic activation of the Unfolded Protein Response (UPR), a common feature among the neurodegenerative diseases where STAU1 is increased, and is directly associated with neuronal death. Here we report that STAU1 is a novel modulator of the UPR, and is required for apoptosis induced by activation of the PERK-CHOP pathway. STAU1 levels increased in response to multiple ER stressors and exogenous expression of STAU1 was sufficient to cause apoptosis through the PERK-CHOP pathway of the UPR. Cortical neurons and skin fibroblasts derived from Stau1-/- mice showed reduced UPR and apoptosis when challenged with thapsigargin. In fibroblasts from SCA2 patients or with ALS-causing TDP-43 and C9ORF72 mutations we found highly increased STAU1 ...Continue Reading

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Mentioned in this Paper

C9orf72 protein, human
Gene Knockdown Techniques
Skin Fibroblast
Response to Endoplasmic Reticulum Stress
Pro-apoptosis
Unfolded Protein Response
TARDBP
HTT
LY6E wt Allele
Nerve Degeneration

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