Staurosporine-induced apoptosis of HPV positive and negative human cervical cancer cells from different points in the cell cycle

Cell Death and Differentiation
B BernardC Mougin

Abstract

In the present study, we compare the sensitivity of CaSki and HeLa cells (HPV positive, wild-type p53) and C33A cells (HPV negative, mutated p53) to a protein kinase inhibitor, the staurosporine (ST). We show that ST can reversibly arrest the three cervical-derived cell lines, either in G1 or in G2/M. Beyond certain ST concentrations or/and over 24 h exposure, the cells underwent apoptosis. This process took place in G1 and G2/M for C33A and CaSki plus HeLa cell lines, respectively. By using an in vitro cell-free system, we demonstrated that cytoplasmic extracts from apoptotic cells were sufficient to induce hallmarks of programmed cell death on isolated nuclei. Moreover, we found that only G2/M cytoplasmic extracts from viable CaSki and HeLa cells supplemented with ST, triggered apoptosis while exclusively G1 cytoplasmic fractions from C33A cells were efficient. Our study describes a possible involvement of the HPV infection or/and p53 status in this different ST-induced apoptosis susceptibility.

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M ScheffnerP M Howley
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H A CrissmanE M Bradbury
Nov 21, 1990·Journal of the National Cancer Institute·G K SchwartzB C Liu
Jun 1, 1989·The New England Journal of Medicine·W C ReevesW E Rawls
May 25, 1989·Nature·A W Murray, M W Kirschner
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Oct 1, 1994·The Journal of Cell Biology·K Schulze-OsthoffP H Krammer
May 1, 1995·The Journal of Experimental Medicine·H NakajimaP A Henkart
Aug 30, 1994·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R T SchimkeR Sharma
Mar 3, 1995·Science·S M CrossB J Reid
Jan 1, 1994·Advances in Cancer Research·K H Vousden
Mar 1, 1994·Journal of Cellular Physiology·F TraganosZ Darzynkiewicz
Apr 1, 1994·Experimental Cell Research·R BertrandY Pommier
Jun 16, 1993·Journal of the National Cancer Institute·M H SchiffmanS Wacholder
May 1, 1996·International Journal of Radiation Biology·E J BernhardW G McKenna
Apr 1, 1996·The Journal of Experimental Medicine·N ZamzamiG Kroemer
Nov 15, 1996·International Journal of Cancer. Journal International Du Cancer·K ButzF Hoppe-Seyler
Feb 16, 1999·Oncogene·M HengstschlägerE Hengstschläger-Ottnad
Aug 10, 1999·Nature·W C HahnR A Weinberg
Aug 19, 1999·The Journal of Pathology·J M WalboomersN Muñoz
Nov 26, 1999·The New England Journal of Medicine·K L WallinJ Dillner

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Citations

May 11, 2012·PloS One·Emilie GaiffeChristiane Mougin
Sep 29, 2004·Annals of Hematology·Yi-Fen WangMiao-Chen Chou
Aug 26, 2009·Journal of Biochemical and Molecular Toxicology·Carolina Alvarez-DelgadoMarco A Cerbón
Jan 13, 2006·Angewandte Chemie·Peter T DanielJulia Schuppan
Jul 15, 2011·Macromolecular Bioscience·Suwan N JayasingheChris J Scotton
Mar 26, 2014·Macromolecular Bioscience·Juan Manuel Cárdenas-MaestreRosario M Sánchez-Martín
Aug 15, 2002·Biochemical and Biophysical Research Communications·Magnus K R SamuelssonSam Okret
Aug 13, 2011·Gynecologic Oncology·Zhaoxia ZhangChristopher M Weghorst
Aug 1, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E Ramesh, Ali A Alshatwi
Feb 21, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peisu ZhangMark P Mattson
Jul 6, 2014·Molecular Biology Reports·Nonkululeko N Mthembu, Lesetja Raymond Motadi
Jan 13, 2004·World Journal of Gastroenterology : WJG·Min-Wen HaYuan Yuan
Sep 24, 2021·Biotechnology and Applied Biochemistry·Nidhi NathRajakishore Mishra

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