PMID: 8962469Nov 29, 1996Paper

Steady-state and time-resolved fluorescence measurements for studying molecular interactions: interaction of a calcium-binding probe with proteins

Biophysical Chemistry
K M HirshfieldL Brand

Abstract

The binding of 2-[(2-bis-[carboxymethyl]amino-5-methylphenoxy)-methyl] 6-methoxy-8-bis[carboxymethyl] aminoquinoline, the fluorescent calcium probe Quin2, to serum albumin and several other proteins has been investigated. Changes in fluorescence emission spectra and fluorescence anisotropy revealed interactions between Quin2 and several proteins including human serum albumin, bovine serum albumin, aldolase, phosphoglucose isomerase, glyceraldehyde-3-phosphate dehydrogenase, and alkaline phosphatase. Protein-probe interactions were inhibited by the presence of calcium. Binding was also measured by resonance energy transfer and gel permeation chromatography. Equilibrium binding constants for Quin2 were quantitated by the application of the recently-developed "SPECTRABIND' program to spectroscopic data (D. Toptygin and L. Brand, Anal. Biochem., 224 (1995) 330-338). Binding of Quin2 to human serum albumin is discussed in terms of the published X-ray crystal structure of human serum albumin (X.M. He and D.C. Carter, Nature, 358 (1992) 209-215).

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Citations

Jun 28, 2000·Analytical Biochemistry·S DespaM Ameloot
Feb 19, 2013·Journal of Hazardous Materials·XiaoYun XieXingGuo Chen
Sep 13, 2005·Journal of Photochemistry and Photobiology. B, Biology·Sujit Kumar GhoshSubhash Chandra Bhattacharya
Aug 15, 2014·Virus Research·Rinki MinakshiFaizan Ahmad
Jul 6, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yuanyuan YueJing Fan
Apr 28, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dana StanMihaela Matache
Oct 17, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Claure Nain LunardiIra M Brinn
May 4, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sumita AnupurathKumaran Rajendran

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