Steady-state fluorescence emission from the fluorescent probe, 5-iodoacetamidofluorescein, bound to hemoglobin

Biochemical and Biophysical Research Communications
R E HirschR L Nagel

Abstract

In the past, fluorescence emission from an extrinsic fluorophore bound to heme-proteins would only be studied with the removal of the heme since fluorescence from the fluorophore could not be detected using right-angle optics. Using front-face fluorometry, a significant steady state emission signal originating from the probe bound to hemoglobin is detected. This is the first report of the detection of extrinsic fluorescence of a probe bound to a heme-protein. We also demonstrate that the extrinsic probe, 5-iodoacetamidofluorescein, is covalently bound to hemoglobin, specifically at beta 93 Cysteine. Ligand binding results in a change in the fluorophore fluorescence intensity as predicted by hemoglobin crystallographic studies. Efficiency of energy transfer measurements are made.

References

Apr 5, 1979·Journal of Molecular Biology·J Baldwin, C Chothia
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R S ZukinD E Koshland
Apr 1, 1979·Analytical Biochemistry·J Eisinger, J Flores
Oct 1, 1969·Biochemistry·J Eisinger
Sep 1, 1973·Analytical Biochemistry·R E BeneschS Yung
Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·L Stryer, R P Haugland
Mar 28, 1980·Biochemical and Biophysical Research Communications·R E HirschR L Nagel
Oct 31, 1983·Biochemical and Biophysical Research Communications·R E HirschR L Nagel
Jan 1, 1980·Photochemistry and Photobiology·B AlpertG Weber
Apr 25, 1980·Biochimica Et Biophysica Acta·N S KosowerH M Ranney

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Citations

Oct 1, 1995·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·S N MuddukrishnaM A Smolenski
Jan 1, 2005·Biophysical Journal·Qiuying ChenRhoda Elison Hirsch
Jun 2, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Henning UlrichJoão Bosco Pesquero
Dec 17, 2002·Blood Cells, Molecules & Diseases·Qiuying ChenRhoda Elison Hirsch

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