PMID: 7082649Apr 13, 1982Paper

Steady-state fluorescence polarization of dansylcadaverine-fibrinogen: evidence for flexibility

R Hantgan


The conformation of fibrinogen in solution has been investigated by steady-state fluorescence polarization measurements. Factor XIIIa has been employed to enzymatically incorporate 1-6 mol of dansylcadaverine/mol of fibrinogen into a specific glutamine residue near the carboxy terminus of the gamma chain and up to two sites on the alpha chain. The fluorescence emission maximum of the labeled protein is shifted to 495 nm (from 538 nm for the fluorophore in solution) and the intensity substantially enhanced, indicating the covalently linked dansyl groups residue in a hydrophobic environment in the interior of the protein. This covalent modification does not interfere with the formation of fibrin, following thrombin activation. Steady-state fluorescence polarization measurements were carried out as a function of temperature and in high viscosity solvents. The fluorescent lifetime of dansylcadaverine-fibrinogen was determined by a phase shift technique. Analysis of the data by the Perrin-Weber treatment yields a rotational relaxation time of 160 ns, considerably faster than any realistic hydrodynamic model of fibrinogen would predict. The results are discussed in terms of segmental flexibility.


Jan 1, 1977·Nucleic Acids Research·D E PulleyblankJ Vinograd
Jan 1, 1978·Macromolecules·M E Carr, J Hermans
Jun 1, 1975·Thrombosis Research·G Hudry-ClergeonM Suscillon
Dec 14, 1967·Journal of Molecular Biology·P Wahl, G Weber
Dec 8, 1972·Annals of the New York Academy of Sciences·L LorandA Gray
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J W Donovan, E Mihalyi
Jun 1, 1969·The Journal of Clinical Investigation·L LorandH L Nossel
Nov 23, 1971·Biochemistry·R Chen, R F Doolittle
Aug 1, 1970·Journal of Molecular Biology·J YguerabideL Stryer
Apr 19, 1968·Biochemical and Biophysical Research Communications·L LorandS Tokura
Mar 1, 1968·Biochemistry·L LorandJ Bruner-Lorand
Jul 22, 1965·Biochimica Et Biophysica Acta·P Johnson, E Mihalyi
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W E FowlerH P Erickson
Aug 15, 1981·Journal of Molecular Biology·R C Williams
Jan 25, 1959·The Journal of Biophysical and Biochemical Cytology·C E HALL, H S Slayter
Sep 10, 1948·Science·K Laki, L Lorand


Jan 19, 1987·Biochimica Et Biophysica Acta·R Hantgan
Jan 1, 1988·Journal of Ultrastructure and Molecular Structure Research·E B HunzikerA Haeberli
Dec 7, 2002·Bioorganic & Medicinal Chemistry·Keizo Matsushita, Yukio Sugiura
Jan 1, 1986·Critical Reviews in Oncology/hematology·A Z Budzynski
Jun 27, 1983·Annals of the New York Academy of Sciences·E F PlowC S Cierniewski
Apr 2, 2005·Analytical Biochemistry·Olga CañadasCristina Casals
Jun 2, 1995·The Journal of Biological Chemistry·B M Gorovits, P M Horowitz
Nov 1, 1991·International Journal of Peptide and Protein Research·C A HasselbacherW R Laws

Related Concepts

BioDex 1
Fluorescence Polarization
Protein Conformation

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Laryngeal Neoplasms

Laryngeal Neoplasms occur in the Larynx and are typically associated with smoking and alcohol consumption. Discover the latest research on Laryngeal Neoplasms here.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.