Steady-state kinetics of the overall oxidative phosphorylation reaction in heart mitochondria. Evidence for linkage of the energy-yielding and energy-consuming steps by freely diffusible intermediates and for an allosteric mechanism of respiratory control at coupling site 2

Journal of Bioenergetics and Biomembranes
C D Stoner

Abstract

The three coupling segments of the respiratory chain of bovine heart mitochondria were examined individually by steady-state kinetic methods to determine whether or not freely diffusible intermediates occur between the energy-yielding and energy-consuming steps involved in the oxidative phosphorylation of extramitochondrial ADP. The principal method employed was the dual inhibitor technique, for which an appropriate model is provided. The results indicate that in accordance with the chemiosmotic theory the intermediate reactants that link the energy-yielding rotenone-sensitive (Site 1), cytochrome bc1 (Site 2), and cytochrome aa3 (Site 3) reactions of the respiratory chain to the energy-consuming ATP synthetase, AdN transport, and Pi transport reactions are freely diffusible (delocalized). Site 2 was found to differ from the others in regard to the mechanism by which the energy-linked respiratory chain reaction is controlled by the energy-consuming steps. Whereas the Site 1 and Site 3 respiratory chain reactions are controlled primarily by the thermodynamic mechanism of reaction reversal, the Site 2 respiratory reaction is controlled primarily by a kinetic mechanism in which an intermediate that links it to the energy-consuming...Continue Reading

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Citations

Jan 1, 1991·The International Journal of Biochemistry·R Moreno-Sánchez, M E Torres-Márquez
Oct 1, 1993·The American Journal of Orthopsychiatry·J C Wade
Apr 1, 2016·Journal of Molecular Modeling·Fu-de RenWen-Jing Shi
Jul 9, 2011·Pharmacological Reviews·Kristiina M HuttunenJarkko Rautio

Related Concepts

S 13
Metazoa
Bos indicus
Cytochrome c Oxidase Subunit VIa
Energy Metabolism
H(+)-Transporting ATP Synthase, Acyl-Phosphate-Linked
Mathematics
Mitochondria, Heart
Multienzyme Complexes
NADH, NADPH Oxidoreductases

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