Steady-state kinetics of the reduction of coenzyme Q analogs by complex I (NADH:ubiquinone oxidoreductase) in bovine heart mitochondria and submitochondrial particles

Biochemistry
R FatoGiorgio Lenaz

Abstract

The reduction kinetics of coenzyme Q (CoQ, ubiquinone) by NADH:ubiquinone oxidoreductase (complex I, EC 1.6.99.3) was investigated in bovine heart mitochondrial membranes using water-soluble homologs and analogs of the endogenous ubiquinone acceptor CoQ10 [the lower homologs from CoQ0 to CoQ3, the 6-pentyl (PB) and 6-decyl (DB) analogs, and duroquinone]. By far the best substrates in bovine heart submitochondrial particles are CoQ1 and PB. The kinetics of NADH-CoQ reductase was investigated in detail using CoQ1 and PB as acceptors. The kinetic pattern follows a ping-pong mechanism; the Km for CoQ1 is in the range of 20 microM but is reversibly increased to 60 microM by extraction of the endogenous CoQ10. The increased Km in CoQ10-depleted membranes indicates that endogenous ubiquinone not only does not exert significant product inhibition but rather is required for the appropriate structure of the acceptor site. The much lower Vmax with CoQ2 but not with DB as acceptor, associated with an almost identical Km, suggests that the sites for endogenous ubiquinone bind 6-isoprenyl- and 6-alkylubiquinones with similar affinity, but the mode of electron transfer is less efficient with CoQ2. The Kmin (kcat/Km) for CoQ1 is 4 orders of ma...Continue Reading

References

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Citations

Jan 23, 1999·BioFactors·G LenazC Bovina
Jul 23, 1999·BioFactors·G LenazG Parenti Castelli
May 24, 2008·Biochemical Pharmacology·L GilleW Gregor
Jan 1, 1997·Molecular Aspects of Medicine·G LenazH Rauchova
Jan 6, 2000·Experimental Gerontology·M DrouetM H Ratinaud
Sep 1, 2007·Biochemistry·Vera G GrivennikovaAndrei D Vinogradov
Sep 15, 2005·The Journal of Antibiotics·M Pilar López-GresaErnesto Estornell
Sep 11, 2009·Antioxidants & Redox Signaling·Giorgio Lenaz, Maria Luisa Genova
Feb 8, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Brian J LindemerMarilyn P Merker
Apr 7, 2011·Anesthesia and Analgesia·Ernst-Bernhard KayserMargaret M Sedensky
Oct 14, 2000·Archives of Biochemistry and Biophysics·J R Tormo, E Estornell
Jan 19, 2016·Biochimica Et Biophysica Acta·Stefan DröseAlexander Galkin
Jan 29, 2016·Biochimica Et Biophysica Acta·Giorgio LenazMaria Luisa Genova
Dec 19, 2012·Analytical Biochemistry·Kátia Klug OliveiraCélia Harumi Tengan
Aug 28, 2009·The International Journal of Biochemistry & Cell Biology·Giorgio Lenaz, Maria Luisa Genova
Jul 30, 2014·Biochimica Et Biophysica Acta·Margit HeiskeJean-Pierre Mazat
Nov 17, 2015·Biochimica Et Biophysica Acta·Andrei D Vinogradov, Vera G Grivennikova
May 9, 2007·Mitochondrion·Giorgio LenazMaria Luisa Genova
Aug 10, 2015·Biochimica Et Biophysica Acta·Vanesa Viviana Galassi, Guilherme Menegon Arantes
Oct 13, 2011·BioFactors·Maria Luisa Genova, Giorgio Lenaz
Feb 20, 2010·Molecular Nutrition & Food Research·Lars GilleAndrey V Kozlov
Jun 9, 2004·Free Radical Biology & Medicine·Marilyn P MerkerSaid H Audi
Mar 10, 2009·Biochimica Et Biophysica Acta·Giorgio Lenaz, Maria Luisa Genova
Oct 26, 2005·Bioorganic & Medicinal Chemistry·Nadia ChahbouneDiego Cortes
May 9, 2002·Biochimica Et Biophysica Acta·Barbara VenturaGiorgio Lenaz

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