Steady-state levels of imported tRNAs in Chlamydomonas mitochondria are correlated with both cytosolic and mitochondrial codon usages.

Nucleic Acids Research
Elizaveta VinogradovaL Marechal-Drouard

Abstract

The mitochondrial genome of Chlamydomonas reinhardtii only encodes three expressed tRNA genes, thus most mitochondrial tRNAs are likely imported. The sharing of tRNAs between chloroplasts and mitochondria has been speculated in this organism. We first demonstrate that no plastidial tRNA is present in mitochondria and that the mitochondrial translation mainly relies on the import of nucleus-encoded tRNA species. Then, using northern analysis, we show that the extent of mitochondrial localization for the 49 tRNA isoacceptor families encoded by the C. reinhardtii nuclear genome is highly variable. Until now the reasons for such variability were unknown. By comparing cytosolic and mitochondrial codon usage with the sub-cellular distribution of tRNAs, we provide unprecedented evidence that the steady-state level of a mitochondrial tRNA is linked not only to the frequency of the cognate codon in mitochondria but also to its frequency in the cytosol, then allowing optimal mitochondrial translation.

References

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Citations

Feb 4, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Trevor Lithgow, André Schneider
Feb 4, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Adrian C BarbrookSarah J Tarr
Jun 30, 2009·Biological Chemistry·Juan D Alfonzo, Dieter Söll
Mar 23, 2011·Annual Review of Biochemistry·André Schneider
Oct 31, 2009·The Journal of Biological Chemistry·Ellen Inga BruskeAndré Schneider
Mar 4, 2015·International Journal of Molecular Sciences·Thalia Salinas-GiegéPhilippe Giegé
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·Morgane MichaudLaurence Maréchal-Drouard
Jan 21, 2014·Biochimie·Jonathan L HuotLaurence Maréchal-Drouard
Jun 23, 2012·Molecular Phylogenetics and Evolution·Elizabeth Rodríguez-SalinasDiego González-Halphen
Sep 10, 2018·Biochemistry. Biokhimii︠a︡·N A VerechshaginaI O Mazunin
Jun 12, 2019·IUBMB Life·Catherine Florentz, Richard Giegé
Mar 7, 2021·Genome Biology and Evolution·Shannon J SibbaldJohn M Archibald
Mar 19, 2020·Mitochondrion·Jessica M Warren, Daniel B Sloan
Aug 27, 2021·Molecular Biology and Evolution·Jessica M WarrenDaniel B Sloan

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Methods Mentioned

BETA
aminoacylation

Software Mentioned

MacBas

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