PMID: 2107748Mar 1, 1990Paper

Steady-state pHi, buffering power, and effect of CO2 in a smooth muscle-like cell line

The American Journal of Physiology
R W Putnam, R D Grubbs

Abstract

Intracellular pH (pHi) was studied in the smooth muscle-like cell line, BC3H-1, using the pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF). The initial pHi measured in 20 mM Na N-2-hydroxyethylpiperazine-N'-2 ethanesulfonic acid-buffered medium [NHB; external pH (pHo) 7.4, 37 degrees C] was 6.89 +/- 0.01 (n = 178). pHi was affected by changes in external pHo, pHi changing by approximately 70% of the change in pHo. The intrinsic buffering power (beta int) of these cells, measured either with NH4Cl or Na propionate pulses, is low for muscle cells, averaging approximately 10 mM/pH unit. Steady-state pHi of BC3H-1 cells in NHB acidified reversibly on exposure to 0.5 mM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS; 1.4 +/- 0.3 x 10(-4) pH/s), 1 mM amiloride (2.0 +/- 0.7 x 10(-4) pH/s), or Na-free solution (8.3 +/- 2.4 x 10(-4) pH/s) and alkalinized upon exposure to Cl-free solutions (9.7 +/- 2.2 x 10(-4) pH/s). Exposure of BC3H-1 cells to CO2-HCO3-buffered solutions resulted in a transient acidification followed by an alkalinization of 0.3-0.4 pH unit to a new steady-state pHi of 7.27 +/- 0.01 (n = 65). This new steady-state pHi acidified very slowly upon exposure to 1 mM amiloride (0.3...Continue Reading

References

Jan 1, 1976·The Journal of General Physiology·W F Boron, P De Weer
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S Ohkuma, B Poole
Jan 1, 1986·Annual Review of Physiology·C C Aickin
Aug 1, 1988·Pflügers Archiv : European journal of physiology·J P Gardner, F P Diecke
Jul 1, 1988·Pflügers Archiv : European journal of physiology·C KorbmacherM Wiederholt
Oct 1, 1985·Pflügers Archiv : European journal of physiology·M Mühleisen, V A Kreye
Dec 1, 1983·The Journal of Physiology·R F AbercrombieA Roos
Feb 1, 1982·The Journal of Cell Biology·R MunsonL Glaser

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Citations

Apr 1, 1992·Journal of Pharmacokinetics and Biopharmaceutics·M V St-PierreK S Pang
Oct 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W P GengK S Pang
Jan 9, 1998·Drug Metabolism Reviews·C L ZimmermanY K Pithavala
Aug 22, 2012·Journal of Vascular Research·Ebbe Boedtkjer, Christian Aalkjaer
Jul 13, 1994·Molecular and Cellular Biochemistry·R D Grubbs, A Walter
Jan 11, 2000·Circulation Research·A FrustaciP Anversa
Aug 1, 1993·Journal of Pharmacokinetics and Biopharmaceutics·I A de Lannoy, K S Pang
Dec 1, 1990·Journal of Pharmacokinetics and Biopharmaceutics·I A de LannoyK S Pang
May 20, 1991·Brain Research. Developmental Brain Research·S Y ChowD M Woodbury
Oct 1, 1996·Journal of Neuroscience Methods·N A RitucciR W Putnam

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