Abstract
Human cytomegalovirus (HCMV) infection of an astrocytoma cell line (U373) or human fibroblast (HF) cells results in a differential cell distribution of the major envelope glycoprotein gB (UL55). This 906-amino-acid type I glycoprotein contains an extracellular domain with a signal sequence, a transmembrane domain, and a 135-amino-acid cytoplasmic tail with a consensus casein kinase II (CKII) site located at Ser900. Since phosphorylation of proteins in the secretory pathway is an important determinant of intracellular trafficking, the state of gB phosphorylation in U373 and HF cells was examined. Analysis of cells expressing wild-type gB and gB with site-specific mutations indicated that the glycoprotein was equally phosphorylated at a single site, Ser900, in both U373 and HF cells. To assess the effect of charge on gB surface expression in U373 cells, Ser900 was replaced with an aspartate (Asp) or alanine (Ala) residue to mimic the phosphorylated and nonphosphorylated states, respectively. Expression of the Asp but not the Ala gB mutation resulted in an increase in the steady-state expression of gB at the plasma membrane (PM) in U373 cells. In addition, treatment of U373 cells with the phosphatase inhibitor tautomycin resulted ...Continue Reading
Citations
Feb 9, 2000·Journal of Virology·T WisnerD C Johnson
Jan 5, 2002·Journal of Virology·Tanja StriveKlaus Radsak
Feb 11, 2004·The Journal of General Virology·Manohara S Mavinakere, Anamaris M Colberg-Poley
Aug 6, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Robert F Murphy
Sep 27, 2013·PLoS Pathogens·James A StahlJ Craig Forrest
Oct 10, 2001·FEBS Letters·H ScheffczikM Eickmann
Sep 28, 2002·Blood·Barry SlobedmanAllison Abendroth
Jan 7, 2000·Journal of Virology·V SanchezW J Britt
Dec 23, 2004·Journal of Virology·Lucie MaresovaCharles Grose
Jan 15, 2004·Journal of Virology·Igor Beitia Ortiz de ZarateFlore Rozenberg
Sep 2, 1999·Nature Medicine·R TomazinD C Johnson
Jun 7, 2002·Journal of Virology·Herman W FavoreelMaurice B Pensaert
Jan 15, 2004·Journal of Virology·Thomas R Jones, Shi-Wu Lee
Feb 14, 2003·Journal of Virology·M Homman-LoudiyiC Söderberg-Nauclér
Sep 23, 1998·The Journal of Cell Biology·S S MolloyG Thomas
Dec 13, 2003·Journal of Virology·Michael A JarvisCarl J Baldick
Mar 9, 2007·Journal of Virology·Maciej T NogalskiAndrew D Yurochko
Apr 23, 2002·Journal of Virology·Michael A JarvisJay A Nelson
Nov 3, 2006·Journal of Virology·Emmanuel J WiertzMaaike E Ressing
Apr 25, 2000·Journal of Virology·A D BrideauL W Enquist
Jun 22, 2000·The Journal of General Virology·A M Colberg-PoleyJ E Slater
Sep 27, 2003·Journal of Virology·Colin M CrumpGary Thomas
Oct 9, 2002·Journal of Virology·T K KenyonCharles Grose