PMID: 9524135May 2, 1998Paper

Steady state relation between cytoplasmic free Ca2+ concentration and force in intact frog skeletal muscle fibers

The Journal of General Physiology
M Konishi, M Watanabe

Abstract

The steady state relation between cytoplasmic Ca2+ concentration ([Ca2+]i) and force was studied in intact skeletal muscle fibers of frogs. Intact twitch fibers were injected with the dextran-conjugated Ca2+ indicator, fura dextran, and the fluorescence signals of fura dextran were converted to [Ca2+]i using calibration parameters previously estimated in permeabilized muscle fibers (Konishi and Watanabe. 1995. J. Gen. Physiol. 106:1123-1150). In the first series of experiments, [Ca2+]i and isometric force were simultaneously measured during high K+ depolarization. Slow changes in [Ca2+]i and force induced by 15-30 mM K+ appeared to be in equilibrium, as instantaneous [Ca2+]i versus force plot tracked the common path in the rising and relaxation phases of K+ contractures. In the second series of experiments, 2,5-di-tert-butylhydroquinone (TBQ), an inhibitor of the sarcoplasmic reticulum Ca2+ pump, was used to decrease the rate of decline of [Ca2+]i after tetanic stimulation. The decay time courses of both [Ca2+]i and force were dose-dependently slowed by TBQ up to 5 micro M; the instantaneous [Ca2+]i- force relations were nearly identical at >/=1 micro M TBQ, suggesting that the change in [Ca2+]i was slow enough to reach equilib...Continue Reading

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Citations

Jul 28, 2001·Clinical and Experimental Pharmacology & Physiology·G S Posterino
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Thomas J Burkholder
Apr 8, 2014·Biomechanics and Modeling in Mechanobiology·J GrasaF J Miana-Mena
Sep 25, 2010·Journal of Theoretical Biology·A RamírezB Calvo
Apr 23, 2008·Current Protocols in Neuroscience·Kerry R Delaney
Jan 13, 2010·Current Protocols in Neuroscience·Kerry R Delaney
Jul 28, 2019·Journal of Muscle Research and Cell Motility·C J Barclay, B S Launikonis

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