Stealth surface modification of surface-enhanced Raman scattering substrates for sensitive and accurate detection in protein solutions

ACS Nano
Fang SunQiuming Yu

Abstract

Reliable surface-enhanced Raman scattering (SERS) based biosensing in complex media is impeded by nonspecific protein adsorptions. Because of the near-field effect of SERS, it is challenging to modify SERS-active substrates using conventional nonfouling materials without introducing interference from their SERS signals. Herein, we report a stealth surface modification strategy for sensitive, specific and accurate detection of fructose in protein solutions using SERS by forming a mixed self-assembled monolayer (SAM). The SAM consists of a short zwitterionic thiol, N,N-dimethyl-cysteamine-carboxybetaine (CBT), and a fructose probe 4-mercaptophenylboronic acid (4-MPBA). The specifically designed and synthesized CBT not only resists protein fouling effectively, but also has very weak Raman activity compared to 4-MPBA. Thus, the CBT SAM provides a stealth surface modification to SERS-active substrates. The surface compositions of mixed SAMs were investigated using X-ray photoelectron spectroscopy (XPS) and SERS, and their nonfouling properties were studied with a surface plasmon resonance (SPR) biosensor. The mixed SAM with a surface composition of 94% CBT demonstrated a very low bovine serum albumin (BSA) adsorption (∼3 ng/cm(2)), ...Continue Reading

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Citations

Jun 7, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Tomas BertokJan Tkac
Jan 16, 2016·Sensors and Actuators. B, Chemical·Samuel S R DasaryAnant K Singh
May 18, 2016·ACS Applied Materials & Interfaces·Ying JiaEugene Pinkhassik
Jun 15, 2016·Nanoscale Research Letters·Mengjing HouZhengjun Zhang
Apr 6, 2017·Chemical Communications : Chem Comm·Fang SunQiuming Yu
Jun 18, 2017·Advanced Materials·Hsiang-Chieh HungShaoyi Jiang
Mar 17, 2017·Annual Review of Analytical Chemistry·Lauren E JamiesonDuncan Graham
Jun 6, 2018·Frontiers in Cellular and Infection Microbiology·Gustavo BodelónIsabel Pastoriza-Santos
Jan 12, 2020·Nature Communications·Nayoung KimMolly M Stevens
May 2, 2018·Current Medicinal Chemistry·Xun ZhangMin-Xuan Sun
Feb 20, 2021·Biosensors & Bioelectronics·Shigekuni HosogiLeonardo Puppulin
Mar 18, 2021·Acta Biomaterialia·Qi ChenRunhui Liu
Nov 2, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Vanessa H FragalRafael Silva
May 1, 2019·ACS Applied Materials & Interfaces·Wei WanJunyong Kang
Aug 23, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Neus FeliuRamon Alvarez-Puebla

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