PMID: 42536Nov 1, 1979

Stearolyl-CoA desaturase: a control enzyme in hepatic lipogenesis

European Journal of Biochemistry
R JeffcoatA T James

Abstract

1. Hepatocytes were isolated by perfusion of the liver with collagenase/salt solutions and incubated in culture after attachment to plastic culture dishes for periods up to 48 h. 2. The cells, when incubated in serum-free culture medium in the presence of insulin, showed enhanced stearolyl-CoA desaturase activity which was not observed when 50 muM cycloheximide was included. When insulin was omitted from the medium, the cells lost 80% of their original desaturase activity. 3. Cells isolated from animals fed 20% (w/w) sucrose for two weeks prior to sacrifice, showed high levels of fatty acid synthesis, stearolyl-CoA desaturase activity and triacylglycerol synthesis when compared with cells isolated from animals fed a corn oil supplemental diet. 4. The observations are discussed in terms of the influence of stearoyl-CoA desaturase activity on hepatic lipogenesis.

References

Apr 1, 1976·Physiological Reviews·J J Volpe, P R Vagelos
Jan 1, 1978·FEBS Letters·R Jeffcoat, A T James
Apr 1, 1972·Archives of Biochemistry and Biophysics·N Oshino, R Sato
Aug 1, 1974·Lipids·P Nilsson-EhleM C Schotz
Jan 1, 1972·Advances in Enzyme Regulation·D M GibsonY Muto
Jan 1, 1973·Lipids·S M Actis DatoR R Brenner
Jan 6, 1970·Biochemical and Biophysical Research Communications·Y Muto, D M Gibson
Nov 1, 1967·Archives of Biochemistry and Biophysics·H G Windmueller, A E Spaeth
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·R G HAM
Jun 1, 1949·Proceedings of the Society for Experimental Biology and Medicine·J H HANKS, R E WALLACE

Citations

Jan 1, 1989·Biological Trace Element Research·S C CunnaneC G Lewis
Jan 1, 1981·Progress in Biophysics and Molecular Biology·P J Quinn
Nov 13, 2013·Expert Opinion on Therapeutic Patents·Alberto LeoniMirella Rambaldi

Related Concepts

Acetic Acids
Dietary Carbohydrates
Ghee
Enzyme Induction
Saturated Fat
Linoleic Acids
Lipids
Liver
Oils
Stearic Acids

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.