Stearoyl-CoA Desaturase-1 Enzyme Inhibition by Grape Skin Extracts Affects Membrane Fluidity in Human Colon Cancer Cell Lines

Nutrients
Valeria TutinoMaria Notarnicola

Abstract

The polyphenolic compounds present in grape extracts have chemopreventive and anticancer properties. Here, we studied the ability of two grape skin extracts (GSEs), Autumn Royal and Egnatia, to influence the cell motility and membrane fluidity regulated by the enzyme Stearoyl-CoA desaturase-1 (SCD1) which increases with the cancer aggressiveness. Caco2 and SW480 human colon cancer cell lines were treated with increasing concentrations of GSEs to evaluate cell proliferation and motility. SCD1 levels were evaluated in both treated cell lines, by membrane lipidomic analysis conducted by gas chromatography. The expression levels of SCD1 and other factors involved in the reorganization of the cytoskeleton and focal adhesions were assessed by Real-time PCR, Western Blotting, and Immunofluorescence staining. High-performance liquid chromatography (HPLC) analyses were performed to determine the phenolic composition in the GSEs, finding them more expressed in Autumn Royal than in Egnatia. Both treatments reduced the levels of SCD1, phospho-Rac1/Cdc42/Rac1/Cdc42 ratio, Cofilin, Vimentin, and phospho-Paxillin especially in Caco2 compared to SW480, showing a different behavior of the two cell lines to these natural compounds. Our findings ...Continue Reading

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Citations

Jul 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valeria TutinoMaria Notarnicola
Oct 22, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·H P Vasantha Rupasinghe
Jul 21, 2021·Nutrition and Cancer·Eric Francisco Contreras-LópezRosa María Oliart-Ros

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Methods Mentioned

BETA
PCR
confocal microscopy

Software Mentioned

GraphPad Prism
ChemStation
STATA
ChromQuest
CFX Manager
Image Lab
Image J

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