STED nanoscopy: a glimpse into the future

Cell and Tissue Research
Paolo BianchiniAlberto Diaspro

Abstract

The well-known saying of "Seeing is believing" became even more apt in biology when stimulated emission depletion (STED) nanoscopy was introduced in 1994 by the Nobel laureate S. Hell and coworkers. We presently stand at a juncture. Nanoscopy represented a revolution in fluorescence microscopy but now is a mature technique applied to many branches of biology, physics, chemistry, and materials science. We are currently looking ahead to the next generation of optical nanoscopes, to the new key player that will arise in the forthcoming years. This article gives an overview of the various cutting-edge implementations of STED nanoscopy and tries to shine a light into the future: imaging everything faster with unprecedented sensitivity and label-free.

References

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Citations

Jan 9, 2016·Journal of Molecular Biology·Karin Nienhaus, G Ulrich Nienhaus
Jan 14, 2016·Scientific Reports·Iván Coto HernándezGiuseppe Vicidomini
Jun 9, 2015·Frontiers in Cellular Neuroscience·Joseph J BrucknerKate M O'Connor-Giles
Mar 17, 2015·Cell and Tissue Research·Christopher S von Bartheld, Fred S Wouters
May 14, 2016·Scientific Reports·Kseniya KorobchevskayaPaolo Bianchini
Jul 7, 2016·Microscopy Research and Technique·Marco CastelloGiuseppe Vicidomini
Aug 2, 2016·Journal of the American Chemical Society·Saumya SaurabhW E Moerner
Jul 26, 2017·Journal of the American Chemical Society·Chenguang WangShigehiro Yamaguchi
Nov 23, 2017·The Journal of Cell Biology·Paolo Armando GagliardiLuca Primo
Jan 30, 2018·Nature Methods·Giuseppe VicidominiAlberto Diaspro
Oct 27, 2015·Scientific Reports·Pietro DelcanaleCristiano Viappiani
Apr 14, 2019·Journal of Biophotonics·Luca PescePaolo Bianchini
May 19, 2020·Journal of Biophotonics·Jialin WangJunle Qu
Sep 9, 2016·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Karin Nienhaus, G Ulrich Nienhaus
Jun 3, 2017·The Review of Scientific Instruments·M CastelloG Vicidomini
Oct 22, 2015·Methods and Applications in Fluorescence·Maksim V SednevStefan W Hell
Apr 1, 2021·Biology of the Cell·Umidahan DjakbarovaCömert Kural
May 14, 2021·Applied Optics·Baby KomalP Senthilkumaran
Aug 2, 2019·ACS Nano·Giulia ZaniniPaolo Bianchini
Apr 18, 2019·Biomacromolecules·Paolo BianchiniCristiano Viappiani
Oct 28, 2021·Microbiology Spectrum·Christine E Broster ReixDerrick R Robinson
Feb 28, 2017··Beniamino BarbieriAlberto Diaspro

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Methods Mentioned

BETA
fluorescence microscopy
optical microscopy
two Hybrid
super-resolution microscopy
Fluorescence correlation spectroscopy
FCS
cross-pair
Fluorescence
electron
atomic force microscopy

Software Mentioned

Picoquant

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