Steel factor directs melanocyte development in vitro through selective regulation of the number of c-kit+ progenitors

Developmental Biology
K ReidM Murphy

Abstract

Studies of mice containing mutations in the genes for a receptor tyrosine kinase, c-kit, or its cognate ligand, Steel factor (SLF), establish that this signaling pathway is required for the development of melanocytes from their precursors in the embryonic neural crest (NC). In order to define the mechanism of this requirement, we have labeled cells expressing c-kit with an anti-c-kit antibody (ACK2) and studied the action of SLF on these cells in cultures of murine trunk NC. c-kit positive (c-kit+) cells first appeared after 2 days in culture and were morphologically indistinguishable from other NC cells. These cells subsequently expressed tyrosinase-related protein, an early marker for the melanocyte lineage, and became pigmented in the presence of a phorbol ester. Further, elimination of the c-kit+ population, by incubating the cultures in ACK2, resulted in the ablation of the melanocyte population, but had no effect on the generation of other neural crest derivatives. These data indicate that c-kit+ cells arising from the neural crest are melanocyte progenitors. The addition of SLF to these cultures stimulated an increase in the number of c-kit+ cells, and further studies indicated that SLF acts as both a survival and a prol...Continue Reading

Citations

Apr 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B Wehrle-Haller, J A Weston
May 23, 2009·The American Journal of Dermatopathology·Stewart F Cramer
Jun 29, 2006·Annual Review of Cell and Developmental Biology·Jennifer F Crane, Paul A Trainor
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·K J DunnW J Pavan
Jan 5, 2002·The Journal of Investigative Dermatology. Symposium Proceedings·T KunisadaS I Hayashi
Aug 12, 1998·The Journal of Investigative Dermatology·J M GrichnikC R Shea
Dec 17, 2008·Seminars in Cell & Developmental Biology·Margaret G Mills, Larissa B Patterson
Jul 31, 2007·Dermatologic Clinics·Karin U Schallreuter
May 17, 2005·Pigment Cell Research·Karen Joyce DunnWilliam J Pavan
Apr 8, 2009·Stem Cells·Tsutomu MotohashiTakahiro Kunisada
Jan 8, 2008·Experimental Dermatology·Karin U SchallreuterJennifer D Spencer
Oct 14, 2006·Stem Cells·Tsutomu MotohashiTakahiro Kunisada
Jun 16, 2001·The Journal of Investigative Dermatology·Y KawaguchiA Nakayama
Apr 3, 2012·Developmental Biology·William J Pavan, David W Raible
Dec 18, 2001·Anais Da Academia Brasileira De Ciências·E DupinN Ledouarin
Jun 6, 2003·Oncogene·Elisabeth Dupin, Nicole M Le Douarin
Sep 24, 2004·Physiological Reviews·Andrzej SlominskiJacobo Wortsman
Jan 14, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T YamaneT Kunisada
Nov 19, 2004·Development·Yvette M WilsonMark Murphy
Apr 1, 1997·Developmental Biology·C S GuoG Ciment
Sep 11, 2004·Development·Nicole M Le DouarinElisabeth Dupin

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