PMID: 7540558Apr 1, 1995Paper

Stem-cell factor in aplastic anemia: in vitro expression in bone marrow stroma and fibroblast cultures

European Journal of Haematology
M S KriegerA Wodnar-Filipowicz

Abstract

In vitro expression of stem-cell factor (SCF) by bone marrow (BM) cells of 30 patients with aplastic anemia (AA) has been analyzed at the mRNA and protein levels. While no deficiencies were found in SCF mRNA expression, low levels of soluble SCF protein were measured in poorly growing AA stroma cultures. The SCF protein concentration in the supernatant and the confluence of AA stroma growth were found to correlate (R = 0.70). Defective proliferation was observed in the majority (20/30) of AA stroma cultures and was paralleled by poor growth of homogeneous cultures of fibroblasts from the same marrow sample. AA stroma growth was enhanced by addition of exogenous SCF in combination with interleukin-11 (IL-11), leukemia inhibitory factor (LIF), and basic fibroblast growth factor (bFGF). Our results demonstrate that deficient growth of stroma cells results in decreased production of SCF. Therefore, SCF and other stroma-derived cytokines may be of therapeutic value in AA patients with documented defects within the BM microenvironment.

References

Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Oct 5, 1990·Cell·D E WilliamsH S Boswell
Jul 1, 1990·British Journal of Haematology·F M Gibson, E C Gordon-Smith
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Nakajima-IijimaT Kakunaga
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N C ZoumbosN S Young
Jan 1, 1985·Acta Haematologica·T HottaH Saito
Mar 1, 1983·British Journal of Haematology·M Y Gordon, E C Gordon-Smith
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S Gartner, H S Kaplan
Mar 18, 1982·The New England Journal of Medicine·B M CamittaE D Thomas

❮ Previous
Next ❯

Citations

Oct 23, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rupasri AinMichael J Soares
Sep 21, 2001·British Journal of Haematology·A Luther-WyrschA Wodnar-Filipowicz
Dec 12, 2001·British Journal of Haematology·J ScopesF M Gibson
Mar 31, 2015·British Journal of Haematology·Edita HamzicRuth Pettengell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.