Stem cell growth factor: in situ hybridization analysis on the gene expression, molecular characterization and in vitro proliferative activity of a recombinant preparation on primitive hematopoietic progenitor cells

The Hematology Journal : the Official Journal of the European Haematology Association
A HiraokaS Sugimoto

Abstract

In situ hybridization of whole mouse fetuses and their tibias with a stem cell growth factor (SCGF) antisense probe demonstrated specific expression of SCGF mRNA around skeletal tissues, particularly in bone marrow cells, proliferating chondrocytes, the perichondrium and periosteum, but little expression in resting or hypertrophic chondrocytes. Recombinant human (rh) SCGF-alpha was purified from a conditioned medium of SCGF-alpha gene-transfected CHO cells. The molecular mass of rhSCGF-alpha, 45 kDa, was shifted down to 40 kDa by digestion with endo-O-glycosidase and sialidase, suggesting O-glycosylation of rhSCGF-alpha with sialic acids. For human bone marrow CD34+Lin- cells, rhSCGF-alpha alone did not stimulate colony-formation, but small cluster-formation (10.3 +/- 2.5/1 x 10(3) CD34+Lin- cells). It promoted growth of erythroid and granulocyte/macrophage (GM) colonies in the primary culture with erythropoietin and GM colony-stimulating factor (CSF) or G-CSF, respectively, and further supported GM progenitor cells in a short-term liquid culture. In contrast, rhSCGF-alpha suppressed stem cell factor (SCF)-stimulated erythroid bursts, indicating some competitive interaction between SCGF and SCF. rhSCGF-alpha was synergistic wit...Continue Reading

Citations

Jun 17, 2009·Infection and Immunity·Christopher C KellerDouglas J Perkins
Sep 29, 2011·Journal of Translational Medicine·Luca Da RivaItalia Bongarzone
Oct 13, 2016·Scientific Reports·Grasiella Angelina AndrianiCristina Montagna
Oct 4, 2014·BMC Immunology·Robert F ForonjyPatrick Geraghty
Sep 25, 2018·BioMed Research International·Caecilia H C SukowatiLory S Crocè
Dec 7, 2018·Frontiers in Genetics·Yaroslav R EfremovSergey S Bogachev
Dec 4, 2004·Birth Defects Research. Part A, Clinical and Molecular Teratology·Partha MukhopadhyayM Michele Pisano
Jun 30, 2018·International Journal of Molecular Medicine·Xiaoyan ZhaoHaibing Wu
Dec 13, 2019·The Journal of Clinical Investigation·Alexandra H MandaranoMaureen R Hanson
Apr 18, 2013·Cancer Research·Sylvie RusakiewiczLaurence Zitvogel

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