Stem cell marker prominin-1/AC133 is expressed in duct cells of the adult human pancreas

Pancreas
Jessy LardonLuc Bouwens

Abstract

Many efforts are spent in identifying stem cells in adult pancreas because these could provide a source of beta cells for cell-based therapy of type 1 diabetes. Prominin-1, particularly its specific glycosylation-dependent AC133 epitope, is expressed on stem/progenitor cells of various human tissues and can be used to isolate them. We, therefore, examined its expression in adult human pancreas. To detect prominin-1 protein, monoclonal antibody CD133/1 (AC133 clone), which recognizes the AC133 epitope, and the alphahE2 antiserum, which is directed against the human prominin-1 polypeptide, were used. Prominin-1 RNA expression was analyzed by real-time polymerase chain reaction. We report that all duct-lining cells of the pancreas express prominin-1. Most notably, the cells that react with the alphahE2 antiserum also react with the AC133 antibody. After isolation and culture of human exocrine cells, we found a relative increase in prominin-1 expression both at protein and RNA expression level, which can be explained by an enrichment of cells with ductal phenotype in these cultures. Our data show that pancreatic duct cells express prominin-1 and surprisingly reveal that its particular AC133 epitope is not an exclusive stem and prog...Continue Reading

References

Apr 1, 1997·Diabetologia·L BouwensR De Krijger
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·A WeigmannW B Huttner
Apr 25, 2000·Biochemical and Biophysical Research Communications·E Hunziker, M Stein
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·S Bonner-WeirJ J O'Neil
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·N UchidaI L Weissman
May 8, 2002·Nature Reviews. Molecular Cell Biology·David Tosh, Jonathan M W Slack
Jun 4, 2002·The Journal of Biological Chemistry·Ying YuJoyce Bischoff
Jul 11, 2002·Histochemistry and Cell Biology·Jessy LardonLuc Bouwens
Jul 13, 2002·The Journal of Pathology·Susan Bonner-Weir, Arun Sharma
Sep 3, 2003·Diabetes/metabolism Research and Reviews·You-Qing Zhang, Nora Sarvetnick
Jan 23, 2004·Diabetes/metabolism Research and Reviews·Andrew M HollandLeonard C Harrison
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Cale N StreetGregory S Korbutt
Jun 9, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jessy LardonLuc Bouwens
Jul 1, 2004·Journal of Cell Science·Gavin D RichardsonAnne T Collins
Nov 19, 2004·Nature·Michael F Clarke
Nov 19, 2004·Nature·Sheila K SinghPeter B Dirks
Feb 1, 2005·The American Journal of Pathology·Benedetta BussolatiGiovanni Camussi
Sep 6, 2005·Differentiation; Research in Biological Diversity·Jessy Lardon, Luc Bouwens
Sep 27, 2005·Physiological Reviews·Luc Bouwens, Ilse Rooman
Nov 23, 2005·Cell Proliferation·S RizzoD L Hudson
Mar 4, 2006·Cell Death and Differentiation·L BaeyensL Bouwens
Dec 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Takuya SugiyamaSeung K Kim

❮ Previous
Next ❯

Citations

Sep 18, 2007·Histochemistry and Cell Biology·József JászaiDenis Corbeil
Jun 10, 2008·Histochemistry and Cell Biology·József JászaiDenis Corbeil
Mar 25, 2010·Histochemistry and Cell Biology·József JászaiDenis Corbeil
Jan 5, 2011·Histochemistry and Cell Biology·Claudia MerkwitzAlbert M Ricken
Mar 1, 2012·Histochemistry and Cell Biology·Stefan Hübner, Athina Efthymiadis
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Liang JinHsun Teresa Ku
May 5, 2009·Stem Cells and Development·Yaojiong Wu, Philip Yuguang Wu
Oct 18, 2008·Diabetes, Obesity & Metabolism·L Baeyens, L Bouwens
Oct 18, 2008·Diabetes, Obesity & Metabolism·T Sugiyama, S K Kim
May 24, 2008·The Journal of Clinical Investigation·Mark A LaBarge, Mina J Bissell
Mar 18, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Heike ImmervollAnders Molven
Jul 23, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jana KarbanováDenis Corbeil
Nov 10, 2010·The Review of Diabetic Studies : RDS·Isabelle Houbracken, Luc Bouwens
Nov 1, 2011·World Journal of Gastroenterology : WJG·Christine A FargeasDenis Corbeil
Dec 12, 2012·Biomarkers in Medicine·Luc Bouwens
Dec 28, 2007·Cells, Tissues, Organs·Nicola BauerDenis Corbeil
Aug 21, 2010·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Rosa CamerlingoGiuseppe Pirozzi
Oct 31, 2015·Diabetes Research and Clinical Practice·Petra AugsteinLeonard C Harrison
Jul 22, 2009·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Atsunori TsuchiyaYutaka Aoyagi
Aug 18, 2012·The Journal of Pathology·Philipp Grosse-GehlingLeoni A Kunz-Schughart
Jun 30, 2012·Seminars in Cell & Developmental Biology·Megan H ClevelandSteven D Leach
Jan 1, 2012·Cancers·Sanne Hindriksen, Maarten F Bijlsma
Jul 3, 2013·Experimental Hematology & Oncology·Zhong Li
Dec 28, 2018·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Tae Hoon RyuKweon Yu
Nov 2, 2014·Clinical Chemistry·Stephen BustinTania Nolan
Feb 14, 2012·Expert Opinion on Therapeutic Targets·Benedetta BussolatiGiovanni Camussi
May 30, 2020·International Journal of Oncology·Nicole A McgrathChangqing Xie
Jul 26, 2011·The International Journal of Artificial Organs·Ali GhodsizadArjang Ruhparwar

❮ Previous
Next ❯

Related Concepts

Related Feeds