Stem cells (SCs) are defined by their combined abilities to both self-renew indefinitely in vitro and differentiate into adult cell types. One of the major driving forces of SC research is that SCs may provide a potentially unlimited source for cell-replacement therapies in regenerative medicine. However, the identification of SCs and their progenies at different stages, and the success of cell-replacement therapies, which form the basis of SC engineering, will depend on the ability to characterize and ultimately isolate homogeneous primary stem or progenitor cell populations to a large degree. Furthermore, the recent identification of cancer stem cells (CSCs) opens a new avenue for developing novel therapeutic strategies by targeting a specific subset of cancer cells with self-renewal and proliferation capacity. Crucial to these tasks will be the discovery of novel plasma membrane-associated SC markers. In this review, we focus on the seminal contribution that membrane proteomics could make to further clinical applications of SCs by providing tools for purification and identification of SCs and their progenies at each stage of differentiation, as well as, to understand the underlying mechanisms of SC differentiation. The need ...Continue Reading
Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum
Neuropathological evidence of graft survival and striatal reinnervation after the transplantation of fetal mesencephalic tissue in a patient with Parkinson's disease
Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms
Immuno-isolation of highly purified peroxisomes using magnetic beads and continuous immunomagnetic sorting
Proteomic analysis of the human colon carcinoma cell line (LIM 1215): development of a membrane protein database
Reducing the risk of colorectal cancer by intervening in the process of carcinogenesis: a status report
Comprehensive proteomic analysis of breast cancer cell membranes reveals unique proteins with potential roles in clinical cancer.
Global profiling of the cell surface proteome of cancer cells uncovers an abundance of proteins with chaperone function.
Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation
Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry
Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues.
Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry.
Capillary high-performance liquid chromatography/mass spectrometric analysis of proteins from affinity-purified plasma membrane
Embryonic stem cell-derived cardiac, neuronal and pancreatic cells as model systems to study toxicological effects
Genomic cis-regulatory architecture and trans-acting regulators of a single interneuron-specific gene battery in C. elegans
Direct proteomic mapping of the lung microvascular endothelial cell surface in vivo and in cell culture
The use of embryonic stem cells for regulatory developmental toxicity testing in vitro--the current status of test development
Improved proteomic profiling of the cell surface of culture-expanded human bone marrow multipotent stromal cells
Expression of the potential cancer stem cell markers, CD133, CD44, ALDH1, and β-catenin, in primary lung adenocarcinoma--their prognostic significance
Tandem application of cationic colloidal silica and Triton X-114 for plasma membrane protein isolation and purification: towards developing an MDCK protein database
Development of cationic colloidal silica-coated magnetic nanospheres for highly selective and rapid enrichment of plasma membrane fractions for proteomics analysis
Adult Stem Cells
Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.
Allogenic & Autologous Therapies
Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.