Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.

Advanced Healthcare Materials
A. L. MichalakKamil Godula

Abstract

Extracellular glycans, such as glycosaminoglycans (GAGs), provide an essential regulatory component during the development and maintenance of tissues. GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface. As such, GAGs have been pursued as biologically active components for the development of biomaterials for cell-based regenerative therapies. However, their structural complexity and compositional heterogeneity make establishing structure-function relationships for this class of glycans difficult. Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network. This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells. With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growt...Continue Reading

References

May 19, 2000·The Journal of Biological Chemistry·L LundinL Claesson-Welsh
Feb 27, 2004·Journal of Biomedical Materials Research. Part a·D SeliktarJ A Hubbell
Mar 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jenny PrestoLena Kjellén
Dec 22, 2009·The Journal of Biological Chemistry·Daniel C KraushaarLianchun Wang
Sep 8, 2010·Methods in Enzymology·Jian Yin, Peter H Seeberger
Jan 27, 2011·Biochemical Society Transactions·Raymond A A SmithCatherine L R Merry
Jun 27, 2017·Journal of the American Chemical Society·Chengli ZongGeert-Jan Boons
Aug 7, 2017·Colloids and Surfaces. B, Biointerfaces·Christina McKee, G Rasul Chaudhry
Oct 29, 2017·Biomaterials·Jungmok SeoAli Khademhosseini
May 2, 2018·Current Opinion in Chemical Biology·Alonso Pardo-VargasPeter H Seeberger
Aug 15, 2018·Nature Methods·Yen-Hsi ChenZhang Yang
Apr 10, 2019·Polymers·Goeun ChoeJae Young Lee
Jun 1, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Lilith M Caballero AguilarSimon E Moulton
Nov 13, 2019·Accounts of Chemical Research·Xing ZhangRobert J Linhardt
Mar 7, 2020·Frontiers in Cell and Developmental Biology·Majid Mossahebi-MohammadiXiaokun Li
Jan 2, 2021·Biomolecules·Harkanwalpreet Sodhi, Alyssa Panitch

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