Stem-Cell Therapy for Esophageal Anastomotic Leakage by Autografting Stromal Cells in Fibrin Scaffold

Stem Cells Translational Medicine
Xiang XueHao Zhang

Abstract

Esophageal anastomotic leakage (EAL) is a devastating complication for esophagectomy but the available therapies are unsatisfactory. Due to the healing effects of mesenchymal stromal cells (MSCs) and supporting capability of fibrin scaffold (FS), we evaluated the efficacy of a stem-cell therapy for EAL by engrafting adult and autologous MSCs (AAMSCs) in FS and investigated the potential mechanism. Twenty-one rabbits were assigned to AAMSC/FS group (n = 12) and control group (n = 9). After harvested, AAMSCs were identified and then labeled with lenti.GFP. To construct EAL model, a polyethylene tube was indwelled through the anastomosis for 1 week. A total of 2 × 106 AAMSCs in 0.2 ml FS were engrafted onto the EAL for the AAMSC/FS group, whereas FS was injected for control. Magnetic Resonance Imaging (MRI) examination was performed after 5 weeks. Esophageal tissues were harvested for macroscopic, histological analyses, Western blot, and immunohistochemistry at 8 weeks. The animal model of EAL was established successfully. MRI scanning revealed a decreased inflammation reaction in AAMSC/FS group. Accordingly, AAMSC/FS group presented a higher closure rate (83.3% vs. 11.1%, p = .02) and lower infection rate (33.3% vs. 88.9%, p = .0...Continue Reading

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Citations

Nov 27, 2019·Macromolecular Bioscience·Iwan Vaughan RobertsNicola Tirelli
Oct 25, 2020·Stem Cell Research & Therapy·Angelo TrivisonnoGabriele Toietta
Aug 8, 2021·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·Joshua Richard BurkeDavid Jayne
Jul 10, 2021·The Journal of Surgical Research·Dana McCloskeyDavid Shersher

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Methods Mentioned

BETA
flow cytometry
Fluorescence microscopy
Protein Assay
transfection

Software Mentioned

Image J
FlowJo
GraphPad Prism

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