PMID: 16639283Apr 28, 2006Paper

Stem cell therapy for hearing loss: Math1 overexpression in VOT-E36 cells

Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology
Jan-Jan LiuJianxin Bao

Abstract

VOT-E36 cells acquire mechanosensitivity after mammalian atonal homolog 1 (Math1) overexpression. VOT-E36 cells are derived from a population of epithelial cells in the ventral region of the otocyst at embryonic Day 10.5, before hair cell differentiation. These cells express a number of specific molecular markers for hair cells under both proliferation and differentiation states. Overexpression of Math1 can convert nonsensory epithelial cells into hair cells in the cochlea. Based on this information, we tested whether VOT-E36 cells can be converted into hair cells by Math1 overexpression. Using reverse transcriptase-polymerase chain reaction-based analysis, we first compared the expression patterns of various molecular markers for hair cell development in VOT-E36 cells between proliferation and differentiation states, and also before and after overexpression of Math1. Subsequently, with a standard calcium imaging method, we examined whether VOT-E36 cells overexpressing Math1 could detect mechanical vibrations and activate spiral ganglion neurons in a coculture model. In addition, using confocal and scanning electron microscopes, we examined morphologic changes of VOT-E36 cells after Math1 overexpression. Consistent with previou...Continue Reading

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Citations

May 2, 2008·Journal of the Association for Research in Otolaryngology : JARO·Hainan LangRichard A Schmiedt
Nov 1, 2012·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Zhaoyu LinJianxin Bao
Oct 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Matthew W Kelley

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