Stem cell transplantation upregulates Sirt1 and antioxidant expression, ameliorating fatty liver in type 2 diabetic mice

International Journal of Biological Sciences
Ming LiSusumu Ikehara

Abstract

Nonalcoholic fatty liver disease (NAFLD) is associated with insulin resistance, oxidative stress, and obesity. The db/db mouse model displays increased levels of insulin resistance, obesity, and an over-accumulation of hepatic triglycerides, making it an excellent model for studying NAFLD. In db/db mice, intra-bone marrow-bone marrow transplantation plus thymus transplantation (IBM-BMT+TT) improves type 2 diabetes mellitus (T2 DM) by normalizing the T-cell imbalance. We hypothesized that this approach would improve Sirt1 expression in the liver and benefit liver development. The db/db mice were treated with IBM-BMT+TT, and plasma MCP-1, IL-6, adiponection, LDL, Sirt1, and HO-1 levels were then assessed. Stem cell transplantation decreased the levels of plasma inflammatory cytokines and LDL while it increased the expression of Sirt1 and HO-1, resulting in decreased progression of fatty liver. Moreover, Sirt1 and HO-1 expression were both detected in the thymus and many HO-1-positive cells were observed in the bone marrow. This is the first report of stem cell transplantation improving the antioxidant function in the liver, thymus, and bone marrow of db/db mice by increasing the levels of Sirt1 and HO-1. This approach may prove u...Continue Reading

Citations

Dec 10, 2015·Journal of Cellular and Molecular Medicine·Ying WanFanyin Meng
Feb 24, 2016·PPAR Research·Luca VanellaIgnazio Barbagallo
Dec 24, 2015·International Journal of Molecular Sciences·Ming LiSusumu Ikehara
May 7, 2020·Journal of Transplantation·Sara AssadiaslNarjes Soleimanifar
Jul 12, 2018·Oxidative Medicine and Cellular Longevity·Mario MasaroneMarcello Persico

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