Stem cells derived from human first-trimester umbilical cord have the potential to differentiate into oocyte-like cells in vitro

International Journal of Molecular Medicine
Xiang HuShang-Mian Yie

Abstract

Compared to stem cells derived from human term umbilical cord, stem cells derived from human first-trimester umbilical cord (hFTUC) exhibit a significantly greater proliferative potential, and more efficiency in terms of their in vitro differentiation. In the present study, we investigated whether hFTUC-derived stem cells are able to differentiate into germ cells. The hFTUC-derived stem cells were first isolated, expanded and then cultured in differentiation medium containing human follicular fluid, follicle-stimulating hormone (FSH)/luteinizing hormone (LH) and estradiol for 24 days. During the period of induction, a subpopulation of the cultured cells appeared that had a morphological resemblance to primordial germ cells (PGCs) and cumulus-oocyte complex (COC)-like cells, and oocyte-like cells (OLCs). The PGC-like cells expressed specific markers indicative of germ cell formation such as octamer-binding transcription factor 4 (OCT4), stage-specific embryonic antigen 1 (SSEA1), B lymphocyte-induced maturation protein-1 (Blimp1), PR domain containing 14 (Prdm14), transcription factor AP-2 gamma (Tfap2C), VASA, STELLA, deleted in azoospermia-like (DAZL) and interferon-induced transmembrane protein 3 (IFITM3). The OLCs, which con...Continue Reading

References

Feb 1, 1994·Biology of Reproduction·J E Fortune
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·D H CastrillonC P Crum
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 6, 2003·Science·Karin HübnerHans R Schöler
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yayoi ToyookaToshiaki Noce
Feb 14, 2004·Human Molecular Genetics·Amander T ClarkRenee A Reijo Pera
May 15, 2004·Human Reproduction·L LefièvreC L R Barratt
Jul 24, 2004·Animal Reproduction Science·R B GilchristD T Armstrong
Oct 16, 2004·EMBO Reports·James KehlerAlexey Tomilin
May 6, 2005·Reproductive Biology and Endocrinology : RB&E·Antonin BukovskyMichael R Caudle
Oct 18, 2005·Journal of Cellular Biochemistry·Katia MareschiFranca Fagioli
Mar 28, 2006·Nature Cell Biology·Paul W DyceJulang Li
Jan 27, 2007·Stem Cells and Development·Kehkooi KeeRenee A Reijo Pera
May 11, 2007·Differentiation; Research in Biological Diversity·Tingting QingHongkui Deng
Nov 21, 2007·Stem Cells·Candace L KerrJohn D Gearhart
Dec 25, 2008·PloS One·Wei WeiHongkui Deng
Jan 31, 2009·Human Reproduction Update·A I Marques-MariC Simón
Nov 9, 2010·Stem Cells and Development·Paul W DyceJulang Li
Dec 7, 2010·Human Molecular Genetics·Sarita PanulaRenee A Reijo Pera
Oct 18, 2011·Current Biology : CB·Li-Fang ChuThomas P Zwaka
Apr 6, 2013·Stem Cells and Development·Seok-Ho HongClifford L Librach
Jul 16, 2013·Nature Cell Biology·Erna MagnúsdóttirM Azim Surani

❮ Previous
Next ❯

Citations

Jul 4, 2017·Zygote : the Biology of Gametes and Early Embryos·José Jackson do Nascimento CostaJosé Roberto Viana Silva
Mar 30, 2017·Expert Opinion on Biological Therapy·Valeria Stella VanniVeronica Giorgione

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ELISA

Software Mentioned

SPSS

Related Concepts

Related Feeds

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.