Stem cells isolated from adult rat muscle differentiate across all three dermal lineages

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Sherri S SchultzPaul A Lucas

Abstract

Adult stem cells capable of differentiating into phenotypes from all three dermal layers were isolated from adult rat muscle. Stem cells were obtained by enzymatic digestion, followed by primary culture in Eagle's minimum essential medium +10% preselected horse serum. When the cells reached confluence, they were released by trypsin, filtered to remove differentiated myotubes, and then slow frozen in 7.5% dimethylsulfoxide to -80 degrees C. Thawed cells were the stem cells and were induced to differentiate with the nonspecific differentiating agent dexamethasone at concentrations of 10(-10)-10(-6) M. After a 6-week treatment with dexamethasone, the cells were assayed by immunohistochemistry for phenotypes of the mesodermal, ectodermal, and endodermal lineages. Examples of mesodermal phenotypes identified were as follows: bone, cartilage, and skeletal, smooth, and cardiac muscle. Ectodermal phenotypes identified were as follows: neurons and oligodendrocytes. Hepatocyte phenotypes identified represented the endodermal lineage. All the phenotypes were observed only with treatment with dexamethasone. However, nestin was observed in the absence of dexamethasone and may be a marker for uncommitted pluripotent stem cells. The results s...Continue Reading

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Solter, B B Knowles
Jan 1, 1990·Cold Spring Harbor Symposia on Quantitative Biology·S HockfieldH Fryer
May 1, 1990·The Journal of Comparative Neurology·B FriedmanS Hockfield
Dec 1, 1982·The Journal of Cell Biology·D BaderD A Fischman
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Mar 1, 1981·Bioscience Reports·J N Wood, B H Anderton
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·J A ThomsonJ P Hearn
Sep 1, 1993·In Vitro Cellular & Developmental Biology. Animal·H E YoungP A Lucas
Aug 1, 1996·Biology of Reproduction·J A ThomsonJ P Hearn
Nov 6, 1998·Science·J A ThomsonJ M Jones
May 15, 1999·Science·B E PetersenJ P Goff
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·G C KopenD G Phinney
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·K A JacksonM A Goodell
Jan 27, 2000·Cell·E Fuchs, J A Segre
Aug 10, 2000·Journal of Neuroscience Research·D WoodburyI B Black
Nov 10, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·F Strutz, G A Müller
Apr 5, 2001·Nature·D OrlicP Anversa
Jun 8, 2001·The Journal of Clinical Investigation·K A JacksonM A Goodell
Jul 13, 2001·Pulmonary Pharmacology & Therapeutics·I M Adcock
Sep 5, 2001·Nature Cell Biology·J G TomaF D Miller
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·G CondorelliG Cossu
Jan 10, 2002·The Journal of Cell Biology·Atsushi SuzukiHideki Taniguchi
Mar 29, 2002·Nature Immunology·Stuart H Orkin, Leonard I Zon
May 8, 2002·Nature Reviews. Molecular Cell Biology·David Tosh, Jonathan M W Slack
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Aug 6, 2002·Experimental Hematology·Yuehua JiangCatherine M Verfaillie
Sep 3, 2002·Journal of Neuroscience Research·Marina Romero-RamosMarie-Françoise Chesselet
Nov 5, 2002·The Journal of Biological Chemistry·Simone de HaijCees van Kooten
Mar 1, 2006·Molecular Cancer Therapeutics·Xiao-Mei LiFrancis Lévi
Oct 1, 1995·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·P A LucasH E Young
Jan 1, 1998·Journal of Cellular Biochemistry·Lawrence J Bonassar, Charles A Vacanti

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Citations

Sep 4, 2008·Plastic and Reconstructive Surgery·Harvey ChimArun K Gosain
Apr 2, 2009·Plastic and Reconstructive Surgery·Peter J TaubPaul A Lucas
Feb 10, 2011·PloS One·Alexander BirbrairOsvaldo Delbono
Jul 17, 2008·Cellular Immunology·Alejandro LealNeil H Riordan
Sep 21, 2007·European Journal of Cell Biology·Jennifer KajahnSandra Danner
Jan 30, 2013·Cell Biology International·Audrone KalvelyteAusra Imbrasaite
Jun 30, 2009·Neurotoxicology and Teratology·Joseph M BreierTimothy J Shafer
May 5, 2009·European Journal of Cell Biology·Erwin GorjupHagen von Briesen
Jan 6, 2009·Gastroenterology·Lynda ElghaziErnesto Bernal-Mizrachi
Aug 16, 2016·Fertility and Sterility·Philippe R KoninckxLeila Adamyan
Jun 17, 2011·Clinical Orthopaedics and Related Research·Scott RussinoffPaul A Lucas
Jul 14, 2007·The International Journal of Artificial Organs·P A Lucas
Sep 8, 2007·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·J GaoA I Caplan
Jul 14, 2007·The International Journal of Artificial Organs·N Di PaoloG Garosi

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