Stem cells isolated from human dental pulp and amniotic fluid improve skeletal muscle histopathology in mdx/SCID mice

Stem Cell Research & Therapy
Alessandra PisciottaA De Pol

Abstract

Duchenne muscular dystrophy (DMD), caused by a lack of the functional structural protein dystrophin, leads to severe muscle degeneration where the patients are typically wheelchair-bound and die in their mid-twenties from cardiac or respiratory failure or both. The aim of this study was to investigate the potential of human dental pulp stem cells (hDPSCs) and human amniotic fluid stem cells (hAFSCs) to differentiate toward a skeletal myogenic lineage using several different protocols in order to determine the optimal conditions for achieving myogenic commitment and to subsequently evaluate their contribution in the improvement of the pathological features associated with dystrophic skeletal muscle when intramuscularly injected into mdx/SCID mice, an immune-compromised animal model of DMD. Human DPSCs and AFSCs were differentiated toward myogenic lineage in vitro through the direct co-culture with a myogenic cell line (C2C12 cells) and through a preliminary demethylation treatment with 5-Aza-2'-deoxycytidine (5-Aza), respectively. The commitment and differentiation of both hDPSCs and hAFSCs were evaluated by immunofluorescence and Western blot analysis. Subsequently, hDPSCs and hAFSCs, preliminarily demethylated and pre-differen...Continue Reading

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Citations

Dec 13, 2016·Journal of Tissue Engineering and Regenerative Medicine·Gianluca CarnevaleAnto de Pol
Jun 27, 2017·Annals of Medicine·João BotelhoJosé João Mendes
Sep 11, 2016·Stem Cell Research & Therapy·Heidi R Hofer, Rocky S Tuan
May 18, 2018·Frontiers in Bioengineering and Biotechnology·Mona K Marei, Rania M El Backly
Oct 2, 2019·Neural Regeneration Research·Alessandra PisciottaGianluca Carnevale
Jul 29, 2017·Stem Cell Research & Therapy·Ester Martínez-SarràMaurilio Sampaolesi
Dec 23, 2017·Frontiers in Physiology·Beatriz A Rodas-JuncoGeovanny I Nic-Can
Jun 2, 2020·Anatomical Science International·Ahmed Othman Fathy Othman El-KershRasha H Al-Serwi
Jun 6, 2020·Frontiers in Cell and Developmental Biology·Alessandra PisciottaGianluca Carnevale
Sep 25, 2020·International Journal of Molecular Sciences·Mohammed Zayed, Koichiro Iohara
Jan 23, 2021·International Journal of Molecular Sciences·Quan-Wen LiuHong-Bo Xin
Jan 27, 2021·Stem Cell Research & Therapy·Yuko Nitahara-KasaharaTakashi Okada
Aug 1, 2018·JACC. Basic to Translational Science·Fiona C LewisGeorgina M Ellison-Hughes
Jan 23, 2021·International Journal of Molecular Sciences·Oana BaruMîndra Badea
Jun 8, 2021·Frontiers in Bioengineering and Biotechnology·Martina SandonàValentina Saccone
Jun 18, 2021·World Journal of Stem Cells·Jing-Yao YinDan-Dan Ma
Jul 3, 2021·Metabolic Brain Disease·Xiaorong ZhaoBo Niu

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Datasets Mentioned

BETA
GM

Methods Mentioned

BETA
fluorescence-activated cell sorting
Protein
electrophoresis
flow cytometry

Software Mentioned

GraphPad Prism
GraphPad
FlowJo
FloMax
Northern Eclipse imaging Imaging
ImageJ
MacOS
Adobe Photoshop

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