Abstract
Mesenchymal stromal cells (MSC) are a promising tool for cellular therapy and regenerative medicine. One major difficulty in establishing a MSC expansion protocol is the large volume of bone marrow (BM) required. We studied whether cells trapped within a collection bag and filter system could be considered as a source of MSC. From the 20 BM collection bag and filter systems, we recovered an average of 1.68 × 108 mononuclear cells, which is the equivalent to 60 ml of filtered BM. Mononuclear cells were expanded ex vivo to 17 × 106 MSC, with purity shown by a CD44+, CD105+, CD90+ and CD73+ immunophenotype, a reduction of 20% proliferating cells in a mixed lymphocyte reaction and also the ability of adipocyte differentiation. Long-term MSC cultures were established from the usually discarded BM collection bag and filter, maintaining an appropriate phenotype and function, being suitable for both investigation and clinical settings.
References
Apr 2, 1999·Science·M F PittengerD R Marshak
Apr 28, 1999·Bone Marrow Transplantation·I BlazsekJ L Misset
Jan 19, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·O N KoçH M Lazarus
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Jun 6, 2006·Bone Marrow Transplantation·D VicenteA Bacigalupo
Aug 23, 2006·Cytotherapy·M DominiciEm Horwitz
Apr 25, 2007·Transplantation·Aly S MageedRichard A West
Aug 8, 2007·Bone Marrow Transplantation·C CapelliM Introna
May 13, 2008·Lancet·Katarina Le BlancUNKNOWN Developmental Committee of the European Group for Blood and Marrow Transplantation
Jun 20, 2008·Cell Biology International·Jana DvorakovaLukas Kubala
Sep 4, 2009·Cytotherapy·Minoo Battiwalla, Peiman Hematti
Sep 11, 2009·Transplantation·Marc H DahlkeUNKNOWN MISOT Study Group
Aug 13, 2010·Nature·Simón Méndez-FerrerPaul S Frenette
Sep 8, 2010·Bone Marrow Transplantation·J Y WengA P Xiang
Dec 3, 2010·Journal of Clinical and Experimental Hematopathology : JCEH·Kazuya SatoKeiya Ozawa
Apr 30, 2011·Stem Cells and Development·Michelle A ScottAaron W James
Jul 7, 2011·PloS One·David M HarrisZeev Estrov
Aug 21, 2012·PloS One·Natalie FeketeHubert Schrezenmeier
Oct 3, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Arthur A NeryHenning Ulrich
Nov 8, 2012·PloS One·Manoj M LaluUNKNOWN Canadian Critical Care Trials Group
Jan 4, 2013·Biological Research·Fernando E FigueroaMaroun Khoury
May 14, 2016·Haematologica·Zyrafete KuçiSelim Kuçi
May 22, 2016·Journal of Translational Medicine·Chantal LechanteurYves Beguin
Feb 13, 2017·Experimental Hematology·Isabel BeermanSimon Méndez-Ferrer
Jul 14, 2017·Transplantation·Marlies E J ReindersJohan W de Fijter
Jan 31, 2018·Bone Marrow Transplantation·Peter BaderSelim Kuçi