Stem domains of heparan sulfate 6-O-sulfotransferase are required for Golgi localization, oligomer formation and enzyme activity

Journal of Cell Science
Naoko NagaiKoji Kimata

Abstract

Heparan sulfate O-sulfotransferases catalyze the O-sulfation of the glucosamine and uronic acid residues of heparan sulfate, thereby determining the binding sites for ligands necessary for important biological functions such as the formation of morphogen gradients and growth factor signaling. Here we investigated the localization of the three heparan sulfate 6-O-sulfotransferase (HS6ST) isoforms and the mechanism of their localization. All three GFP-tagged HS6STs localized in the Golgi apparatus. C-5 epimerase and HS2ST have been shown to form complexes that facilitate their localization in the Golgi but we found that the absence of HS2ST did not alter the localization of any of the HS6STs. Neither the forced expression of HS2ST in the rough endoplasmic reticulum (ER), the deletion of most of the lumenal domain nor increasing the length of the transmembrane domain had any effect on the localization of HS6STs. However, deletions in the stem region did affect the Golgi localization of the HS6STs and also reduced their sulfotransferase activity and oligomer formation. These findings suggest that the stem region of HS6ST plays an important role in normal functioning, including the transit of HS6ST to the Golgi apparatus and maintai...Continue Reading

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Citations

Mar 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jenny PrestoLena Kjellén
Aug 18, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hinke A B Multhaupt, John R Couchman
Sep 4, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Gunnar DickKristian Prydz
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Nov 1, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Adam B Cadwallader, H Joseph Yost
Nov 5, 2016·Glycoconjugate Journal·Rana El MasriRomain R Vivès
Mar 17, 2007·The Journal of Biological Chemistry·Naoko NagaiKoji Kimata
May 20, 2008·The Journal of Biological Chemistry·Pernilla CarlssonLena Kjellén
Aug 9, 2018·Scientific Reports·David F ThiekerRobert J Woods
Apr 2, 2011·The Journal of Biological Chemistry·Robert A Townley, Hannes E Bülow
May 11, 2018·The Journal of Biological Chemistry·Jonathan W MuellerWiebke Arlt
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Sep 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thibault AnnavalHugues Lortat-Jacob
Mar 4, 2021·Nature Communications·Abinaya BadriMattheos A G Koffas

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