Stem rust spores elicit rapid RPG1 phosphorylation

Molecular Plant-microbe Interactions : MPMI
Jayaveeramuthu NirmalaA Kleinhofs

Abstract

Stem rust threatens cereal production worldwide. Understanding the mechanism by which durable resistance genes, such as Rpg1, function is critical. We show that the RPG1 protein is phosphorylated within 5 min by exposure to spores from avirulent but not virulent races of stem rust. Transgenic mutants encoding an RPG1 protein with an in vitro inactive kinase domain fail to phosphorylate RPG1 in vivo and are susceptible to stem rust, demonstrating that phosphorylation is a prerequisite for disease resistance. Protein kinase inhibitors prevent RPG1 phosphorylation and result in susceptibility to stem rust, providing further evidence for the importance of phosphorylation in disease resistance. We conclude that phosphorylation of the RPG1 protein by the kinase activity of the pK2 domain induced by the interaction with an unknown pathogen spore product is required for resistance to the avirulent stem rust races. The pseudokinase pK1 domain is required for disease resistance but not phosphorylation. The very rapid phosphorylation of RPG1 suggests that an effector is already present in or on the stem rust urediniospores when they are placed on the leaf surface. However, spores must be alive, as determined by their ability to germinate,...Continue Reading

References

Jul 1, 1997·Genes & Development·Y YangD F Klessig
May 23, 1998·Annual Review of Immunology·W J Leonard, J J O'Shea
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Zhang, D F Klessig
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Zhang, D F Klessig
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·D C BoyesJ L Dangl
Mar 6, 1999·Current Opinion in Plant Biology·D Scheel
Mar 6, 1999·Current Opinion in Plant Biology·M H RichbergJ L Dangl
May 22, 2001·The Plant Journal : for Cell and Molecular Biology·M R Swiderski, R W Innes
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G FelixT Boller
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
Jun 1, 2002·Science·David S Aaronson, Curt M Horvath
Jun 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·R BrueggemanA Kleinhofs
Aug 2, 2002·Cell·Kyoung Hee Nam, Jianming Li
Jan 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Henriette HorvathAndris Kleinhofs
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Kim E. Hammond-Kosack, Jonathan D. G. Jones
Jul 19, 2005·Plant Physiology·Antje HeeseJonathan D G Jones
May 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jayaveeramuthu NirmalaAndris Kleinhofs
Jul 13, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·L ZhangA Kleinhofs
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Feb 24, 2007·Molecular & Cellular Proteomics : MCP·Joris J BenschopFrank L H Menke
Jun 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jayaveeramuthu NirmalaAndris Kleinhofs
Jul 13, 2007·Nature·Delphine ChinchillaThomas Boller
Jul 27, 2007·The Plant Journal : for Cell and Molecular Biology·Thomas S NühseScott C Peck
Apr 22, 2008·Annual Review of Phytopathology·Vera Göhre, Silke Robatzek
Aug 30, 2008·The Plant Cell·Renier A L van der Hoorn, Sophien Kamoun
Sep 11, 2008·Cellular Microbiology·Caroline Hoefle, Ralph Hückelhoven
Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·R BrueggemanA Kleinhofs
Apr 30, 2009·Annual Review of Phytopathology·Ioannis Stergiopoulos, Pierre J G M de Wit
Oct 24, 2009·Molecular Plant Pathology·Pierre J G M De WitIoannis Stergiopoulos
Nov 3, 2009·Nucleic Acids Research·Pawel DurekWaltraud X Schulze

❮ Previous
Next ❯

Citations

Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jayaveeramuthu NirmalaAndris Kleinhofs
Feb 7, 2014·Molecular Plant-microbe Interactions : MPMI·Scot Hulbert, Michael Pumphrey
Mar 13, 2012·Annual Review of Plant Biology·Benjamin Schwessinger, Pamela C Ronald
Jun 19, 2012·Current Opinion in Plant Biology·Jacqueline Monaghan, Cyril Zipfel
Aug 19, 2015·Frontiers in Plant Science·Melania FigueroaJames M Bradeen
Jan 8, 2015·Frontiers in Plant Science·Jan BettgenhaeuserMatthew J Moscou
May 5, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Upinder GillAndris Kleinhofs
Mar 25, 2016·Molecular Plant Pathology·Mayra C da C G de CarvalhoFrancismar C Marcelino-Guimarães
Mar 14, 2018·Molecular Plant-microbe Interactions : MPMI·Vittorio Nicolis, Eduard Venter
May 30, 2019·Scientific Reports·Shyam SolankiRobert S Brueggeman
Dec 21, 2018·Frontiers in Plant Science·Roshan Sharma PoudelRobert Brueggeman
Jun 8, 2021·Molecular Plant-microbe Interactions : MPMI·Valentyna KlymiukCurtis J Pozniak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Jayaveeramuthu NirmalaA Kleinhofs
Proceedings of the National Academy of Sciences of the United States of America
Jayaveeramuthu NirmalaA Kleinhofs
Annual Review of Plant Biology
Benjamin Schwessinger, Pamela C Ronald
© 2021 Meta ULC. All rights reserved