Stemness-Attenuating miR-503-3p as a Paracrine Factor to Regulate Growth of Cancer Stem Cells

Stem Cells International
Minkoo SeoMihue Jang

Abstract

Cancer stem cells (CSCs) with self-renewal abilities endorse cellular heterogeneity, resulting in metastasis and recurrence. However, there are no promising therapeutics directed against CSCs. Herein, we found that miR-503-3p inhibited tumor growth via the regulation of CSC proliferation and self-renewal. miR-503-3p, isolated from human adipose stem cell- (ASC-) derived exosomes, suppressed initiation and progression of CSCs as determined by anchorage-dependent (colony formation) and anchorage-independent (tumorsphere formation) assays. The expression of pluripotency genes was significantly decreased in miR-503-3p-treated CSCs. Furthermore, xenografts, which received miR-503-3p, exhibited remarkably reduced tumor growth in vivo. Thus, miR-503-3p may function as a stemness-attenuating factor via cell-to-cell communications.

References

Nov 2, 2001·Nature·T ReyaI L Weissman
Aug 3, 2002·Nature Reviews. Immunology·Clotilde ThérySebastian Amigorena
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Mar 12, 2004·Genes & Development·John G Doench, Phillip A Sharp
Oct 19, 2005·Cancer Research·Dong FangMeenhard Herlyn
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Sep 12, 2008·Nature Reviews. Cancer·Jane E Visvader, Geoffrey J Lindeman
Mar 2, 2010·Cell Cycle·Fadila GuessousRoger Abounader
Sep 30, 2010·Cancer Investigation·Mark KatakowskiMichael Chopp
Sep 3, 2011·Breast Cancer Research : BCR·Deborah L Holliday, Valerie Speirs
Oct 25, 2011·Nature Cell Biology·Yong Jin ChoiLin He
Dec 6, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Susan M van DommelenRaymond M Schiffelers
Oct 5, 2012·Stem Cells and Development·Stefania BrunoGiovanni Camussi
Feb 20, 2013·Cancer Letters·Mark KatakowskiMichael Chopp
Mar 23, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Christoph Kahlert, Raghu Kalluri
Apr 16, 2013·Nature Reviews. Drug Discovery·Samir EL AndaloussiMatthew J A Wood
Sep 18, 2013·Stem Cell Research & Therapy·Hong-Yo Kang
Sep 18, 2013·OncoTargets and Therapy·Mong-Lien WangCheng-Wen Wu
Jan 31, 2014·Expert Review of Proteomics·Crislyn D'Souza-Schorey, Dolores Di Vizio
Feb 26, 2014·Nature Reviews. Immunology·Paul D Robbins, Adrian E Morelli
Aug 27, 2014·Frontiers in Immunology·Stefania BrunoGiovanni Camussi
Dec 30, 2014·Expert Opinion on Biological Therapy·Andrea Del FattoreMaurizio Muraca
Mar 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Marc A Antonyak, Richard A Cerione
Dec 10, 2015·Current Stem Cell Research & Therapy·Il-Kwon KimHan-Soo Kim
Jan 23, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shentong YuJian Zhang
Oct 31, 2016·Advanced Drug Delivery Reviews·Pieter VaderRaymond M Schiffelers
Apr 14, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Stephan StremerschKoen Raemdonck
Jun 22, 2016·Cancer Research·Alexander A SvoronosFrank J Slack
Jan 28, 2017·Frontiers in Pharmacology·Jinheng WangMeng Zhao
May 20, 2017·Oncology Letters·Junyi WuChun-Ping Jiang
Aug 12, 2017·Cancer Biomarkers : Section a of Disease Markers·Yi SunQiang Shen

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Citations

Jul 7, 2019·International Journal of Molecular Sciences·Maria Giovanna ScioliAugusto Orlandi
Jul 13, 2021·Frontiers in Oncology·Haoying WangYongning Zhou

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Methods Mentioned

BETA
PCR
Assay
xenografts
confocal microscopy
RNA-Seq

Software Mentioned

Nanoparticle Tracking Analysis
ImageJ
AGCC
GeneChip Command Console®
Affymetrix®

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