Stem/progenitor cells in non-lactating versus lactating equine mammary gland

Stem Cells and Development
Jan H SpaasGerlinde R Van de Walle

Abstract

The mammary gland is a highly regenerative organ that can undergo multiple cycles of proliferation, lactation, and involution. Based on the facts that (i) mammary stem/progenitor cells (MaSC) are proposed to be the driving forces behind mammary growth and function and (ii) variation exists between mammalian species with regard to physiological and pathological functioning of this organ, we believe that studying MaSC from different mammals is of great comparative interest. Over the years, important data has been gathered on MaSC of men and mice, although knowledge on MaSC in other mammals remains limited. Therefore, the aim of this work was to isolate and characterize MaSC from the mammary gland of horses. Hereby, our salient findings were that the isolated equine cells met the 2 in vitro hallmark properties of stem cells, namely the ability to self-renew and to differentiate into multiple cell lineages. Moreover, the cells were immunophenotyped using markers for CD29, CD44, CD49f, and Ki67. Finally, we propose the mammosphere assay as a valuable in vitro assay to study MaSC during different physiological phases since it was observed that equine lactating mammary gland contains significantly more mammosphere-initiating cells tha...Continue Reading

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Citations

Jan 31, 2013·Stem Cells and Development·Bizunesh M BorenaGerlinde R Van de Walle
Mar 12, 2015·Journal of Biomolecular Structure & Dynamics·Neelesh SharmaDong Kee Jeong
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Oct 1, 2020·Radiation and Environmental Biophysics·Ayaka HosokiTatsuhiko Imaoka
Oct 14, 2016·The Journal of Biological Chemistry·Leen BusscheGerlinde R Van de Walle
Aug 24, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Gat RaunerGerlinde R Van de Walle
Oct 29, 2020·Cancer Metastasis Reviews·Rebecca M HarmanGerlinde R Van de Walle
Nov 27, 2020·Scientific Reports·Melissa M LedetGerlinde R Van de Walle
Dec 7, 2020·Journal of Mammary Gland Biology and Neoplasia·Katherine Hughes

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Methods Mentioned

BETA
flow cytometry
FACS
SMA
light microscopy

Software Mentioned

FACS Diva
Image J

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