Step-wise and punctuated genome evolution drive phenotype changes of tumor cells

Mutation Research
Aleksei StepanenkoVadym Kavsan

Abstract

The pattern of genome evolution can be divided into two phases: the step-wise continuous phase (step-wise clonal evolution, stable dominant clonal chromosome aberrations (CCAs), and low frequency of non-CCAs, NCCAs) and punctuated phase (marked by elevated NCCAs and transitional CCAs). Depending on the phase, system stresses (the diverse CIN promoting factors) may lead to the very different phenotype responses. To address the contribution of chromosome instability (CIN) to phenotype changes of tumor cells, we characterized CCAs/NCCAs of HeLa and HEK293 cells, and their derivatives after genotoxic stresses (a stable plasmid transfection, ectopic expression of cancer-associated CHI3L1 gene or treatment with temozolomide) by conventional cytogenetics, copy number alterations (CNAs) by array comparative genome hybridization, and phenotype changes by cell viability and soft agar assays. Transfection of either the empty vector pcDNA3.1 or pcDNA3.1_CHI3L1 into 293 cells initiated the punctuated genome changes. In contrast, HeLa_CHI3L1 cells demonstrated the step-wise genome changes. Increased CIN correlated with lower viability of 293_pcDNA3.1 cells but higher colony formation efficiency (CFE). Artificial CHI3L1 production in 293_CHI3...Continue Reading

References

Nov 8, 2005·Cancer Genetics and Cytogenetics·Ruhong LiPeter Duesberg
Jan 26, 2006·Cancer Genetics and Cytogenetics·Paul WinnardVenu Raman
Apr 11, 2006·Genome Génome / Conseil National De Recherches Canada·Henry H Q HengChristine J Ye
Mar 28, 2007·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Peter DuesbergRuediger Hehlmann
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·ChongFeng GaoGeorge F Vande Woude
Feb 12, 2008·Surgical Neurology·Brian T RagelRandy L Jensen
Dec 31, 2008·Journal of Cellular Physiology·Christine J YeHenry H Q Heng
May 15, 2009·Journal of Cellular Physiology·Henry H Q HengChristine J Ye
Sep 29, 2009·Cancer Genetics and Cytogenetics·Joshua M Nicholson, Peter Duesberg
Mar 10, 2010·Journal of Cellular Biochemistry·Henry H Q HengChristine J Ye
Jul 14, 2010·Cancer Genetics and Cytogenetics·Andreas KleinPeter Duesberg
Nov 6, 2010·Medical Hypotheses·Jonathan M Lee
Jan 29, 2011·Cancer Research·Nicolai J BirkbakCharles Swanton
Mar 3, 2011·Cancer Research·Alvin J X LeeCharles Swanton
Jun 15, 2011·Cell Cycle·Peter DuesbergJoshua M Nicholson
Jul 26, 2011·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Rebecca RoylanceCharles Swanton
Mar 20, 2012·Biochimica Et Biophysica Acta·Thomas RiedJordi Camps
May 9, 2012·Journal of Neurochemistry·Caroline HappoldMichael Weller
Aug 23, 2012·Frontiers in Oncology·Mitsutoshi NakadaJun-Ichiro Hamada
Sep 29, 2012·Journal of Cellular Physiology·Steven D HorneHenry H Q Heng
Apr 19, 2013·Cancer Metastasis Reviews·Joshua B StevensHenry H Heng
Apr 23, 2013·Cancer Metastasis Reviews·Henry H HengChristine J Ye
Aug 13, 2013·Gene·A A StepanenkoV M Kavsan
Oct 15, 2013·International Journal of Cancer. Journal International Du Cancer·Joshua B StevensHenry H Heng
Oct 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Alain D SilkBeth A Weaver
Oct 19, 2013·Molecular Cytogenetics·Amanda McCormackPeter Duesberg
Nov 2, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Violetta V LeshchenkoSamir Parekh
Dec 4, 2013·Biochimica Et Biophysica Acta·Neysan Donnelly, Zuzana Storchová
Dec 5, 2013·Cell Cycle·Guo LiuHenry H Heng
Mar 25, 2014·Current Opinion in Genetics & Development·Rebecca A Burrell, Charles Swanton
Apr 3, 2014·The International Journal of Biochemistry & Cell Biology·Nipaporn NgernyuangTemduang Limpaiboon

❮ Previous
Next ❯

Citations

Feb 16, 2016·Molecular Cytogenetics·Henry H Q HengChristine J Ye
Dec 3, 2015·Molecular Cytogenetics·Tamara BorgonovoPaulo Slud Brofman
May 10, 2016·Cancer Cell International·Aleksei A StepanenkoVladimir V Dmitrenko
Aug 26, 2018·Biotechnology and Bioengineering·Arpan A BandyopadhyayWei-Shou Hu
Jan 27, 2018·Molecular Cytogenetics·Mathew Bloomfield, Peter Duesberg
Nov 8, 2020·Current Genetics·Lydia R HeasleyJuan Lucas Argueso
May 17, 2021·Progress in Biophysics and Molecular Biology·Julie Heng, Henry H Heng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.