PMID: 7541844Aug 1, 1995Paper

Steps in maturation of influenza A virus neuraminidase

Journal of Virology
T SaitoR G Webster

Abstract

We have studied the maturation of the influenza A virus neuraminidase (NA), using monoclonal antibodies (MAbs) with different conformational specificities against the head domains of the N8 NA. The results obtained with radioimmunoprecipitation, together with previously published information, suggest the following steps in maturation of this molecule. First, the folding of the nascent NA leads to formation of the epitope recognized by MAb N8-10, a step that depends on the formation of intramolecular disulfide bonds. Second, monomers form dimers by an intermolecular disulfide linkage in the stalk, with a t1/2 of 2.5 min. Third, the epitope recognized by MAb N8-82 appears after dimerization, suggesting that oligomeric NAs may undergo conformational change with a t1/2 of 8 min. Finally, a tetramer-specific epitope recognized by MAb N8-4 appears on the NA with a t1/2 of 13 min. Epitope detection by MAb N8-4 was inhibited by tunicamycin treatment, suggesting that glycosylation of this molecule is required for proper tetramerization. Each of these proposed steps occurs in the endoplasmic reticulum of host cells, as demonstrated by treatment of virus-infected cells with brefeldin A or carbonyl cyanide m-chlorophenylhydrazine; subseque...Continue Reading

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Citations

Jul 25, 2000·The Journal of Veterinary Medical Science·T Saito, I Yamaguchi
Nov 6, 2012·Nihon Jibiinkoka Gakkai kaiho·Reiko Sato
Sep 17, 2014·The Journal of Antimicrobial Chemotherapy·S HussainJ W McCauley
Nov 15, 1997·The Journal of Veterinary Medical Science·T Saito, K Kawano
May 28, 2010·The Journal of General Virology·Jimmy C C LaiJean-Michel Garcia
Nov 17, 2011·Influenza and Other Respiratory Viruses·Gillian M Air
Nov 15, 2012·The Journal of Biological Chemistry·Diogo V da SilvaRobert Daniels
Oct 26, 1999·Viral Immunology·S LudwigT Wolff
Aug 7, 2018·Frontiers in Immunology·Dan DouRobert Daniels
Dec 16, 2018·Bioscience Reports·Ian A YorkIrina V Alymova
Sep 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Beryl Mazel-SanchezMirco Schmolke

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