Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells.

The Journal of Cell Biology
Xavier DarzacqU T Meier

Abstract

Mammalian H/ACA RNPs are essential for ribosome biogenesis, premessenger RNA splicing, and telomere maintenance. These RNPs consist of four core proteins and one RNA, but it is not known how they assemble. By interrogating the site of H/ACA RNA transcription, we dissected their biogenesis in single cells and delineated the role of the non-core protein NAF1 in the process. NAF1 and all of the core proteins except GAR1 are recruited to the site of transcription. NAF1 binds one of the core proteins, NAP57, and shuttles between nucleus and cytoplasm. Both proteins are essential for stable H/ACA RNA accumulation. NAF1 and GAR1 bind NAP57 competitively, suggesting a sequential interaction. Our analyses indicate that NAF1 binds NAP57 and escorts it to the nascent H/ACA RNA and that GAR1 then replaces NAF1 to yield mature H/ACA RNPs in Cajal bodies and nucleoli.

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Methods Mentioned

BETA
immunoprecipitation
transfection
nuclear translocation
fluorescence microscopy
ChIPs
ChIP
dissection

Software Mentioned

ImageJ
Adobe
Photoshop
IPLab Spectrum

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