Stereochemical conversion of C3-vinyl group to 1-hydroxyethyl group in bacteriochlorophyll c by the hydratases BchF and BchV: adaptation of green sulfur bacteria to limited-light environments

Molecular Microbiology
Jiro HaradaHitoshi Tamiaki

Abstract

Photosynthetic green sulfur bacteria inhabit anaerobic environments with very low-light conditions. To adapt to such environments, these bacteria have evolved efficient light-harvesting antenna complexes called as chlorosomes, which comprise self-aggregated bacteriochlorophyll c in the model green sulfur, bacterium Chlorobaculum tepidum. The pigment possess a hydroxy group at the C3(1) position that produces a chiral center with R- or S-stereochemistry and the C3(1) -hydroxy group serves as a connecting moiety for the self-aggregation. Chlorobaculum tepidum carries the two possible homologous genes for C3-vinyl hydratase, bchF and bchV. In the present study, we constructed deletion mutants of each of these genes. Pigment analyses of the bchF-inactivated mutant, which still has BchV as a sole hydratase, showed higher ratios of S-epimeric bacteriochlorophyll c than the wild-type strain. The heightened prevalence of S-stereoisomers in the mutant was more remarkable at lower light intensities and caused a red shift of the chlorosomal Qy absorption band leading to advantages for light-energy transfer. In contrast, the bchV-mutant possessing only BchF showed a significant decrease of the S-epimers and accumulations of C3-vinyl BChl c...Continue Reading

References

May 1, 1995·Journal of Bacteriology·T M Wahlund, M T Madigan
Apr 15, 1994·Journal of Molecular Biology·D W BollivarC E Bauer
May 26, 2001·Applied and Environmental Microbiology·N U Frigaard, D A Bryant
Aug 14, 1992·Journal of Photochemistry and Photobiology. B, Biology·T P CausgroveR E Blankenship
Feb 6, 2002·Chembiochem : a European Journal of Chemical Biology·T MiyatakeH Tamiaki
Sep 27, 2002·Chembiochem : a European Journal of Chemical Biology·Henning D MootzMohamed A Marahiel
Mar 27, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Tadashi MizoguchiHitoshi Tamiaki
Aug 27, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hitoshi Tamiaki
Oct 18, 2005·Photosynthesis Research·Asunción Martinez-PlanellsJesús Garcia-Gil
Sep 26, 2006·Trends in Microbiology·Donald A Bryant, Niels-Ulrik Frigaard
May 18, 2007·Annual Review of Microbiology·Aline Gomez Maqueo Chew, Donald A Bryant
Oct 11, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jiro HaradaHirozo Oh-oka
May 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Swapna GanapathyHuub J M de Groot
Feb 5, 2010·Photosynthesis Research·Gert T OostergetelEgbert J Boekema
May 10, 2011·The Journal of Biological Chemistry·Zhenfeng Liu, Donald A Bryant
Aug 22, 2012·Frontiers in Microbiology·Kajetan VoglDonald A Bryant
Sep 21, 2012·Scientific Reports·Jiro HaradaHitoshi Tamiaki
Jan 29, 2013·Biochimica Et Biophysica Acta·Gregory S OrfRobert E Blankenship
Jun 14, 2013·Photosynthesis Research·Gregory S Orf, Robert E Blankenship
Oct 23, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Yusuke TsukataniHitoshi Tamiaki
Feb 11, 2015·International Journal of Systematic and Evolutionary Microbiology·Marcus Tank, Donald A Bryant

❮ Previous
Next ❯

Citations

Jan 5, 2020·Chembiochem : a European Journal of Chemical Biology·Mitsuaki HiroseHitoshi Tamiaki
Apr 4, 2020·The Journal of Biological Chemistry·Donald A BryantMartin J Warren
Sep 6, 2018·Photosynthesis Research·Misato TeramuraHitoshi Tamiaki
Mar 3, 2018·Biochimica Et Biophysica Acta. Bioenergetics·Marcia Ortega-RamosJohn H Golbeck

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.