PMID: 8612648Mar 1, 1996Paper

Stereochemical course and reaction products of the action of beta-xylosidase from Thermoanaerobacterium saccharolyticum strain B6A-RI

European Journal of Biochemistry
S ArmandB Henrissat

Abstract

Beta-Xylosidases are grouped in families 39 and 43 of a general classification of glycosyl hydrolases based on amino acid sequence similarities. The Beta-xylosidase from Butyrivibrio fibrisolvens, which belongs to family 43, has been shown to operate by a molecular mechanism which results in the inversion of the anomeric configuration. Thermoanaerobacterium saccharolyticum B6A-RI Beta-xylosidase which belongs to family 39 was purified as a recombinant enzyme from Escherichia coli. The stereochemistry of the hydrolysis of p-nitrophenyl Beta-D-xylopyranoside was followed by 1H NMR. The spectrum recorded after 2 h hydrolysis showed a large signal centred at 4.47 ppm (J approximately 10 Hz) assignable to H1 of free Beta-xylose with a small amount of alpha-xylose (5.05 ppm, J approximately 3 Hz) attributable to mutarotation. This result indicates that T. saccharolyticum Beta-xylosidase operates with overall retention of the anomeric configuration. This result, with the lack of sequence similarity between the two families of Beta-xylosidases, suggests that these two families have major differences in their active-site geometries. Consistent with its retaining mechanism, Beta-xylosidase of T. saccharolyticum B6A-RI also displayed tran...Continue Reading

References

Apr 1, 1976·Carbohydrate Research·H Kersters-HildersonC K de Bruyne
Mar 1, 1985·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·F DeleynA De Bruyn
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·B HenrissatG Davies

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Citations

Aug 10, 2006·Applied Microbiology and Biotechnology·Issam SmaaliMichael J O'Donohue
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·C Vieille, G J Zeikus
Sep 17, 2013·Nature Chemical Biology·Haiying BieMichael N G James
Oct 11, 2005·Journal of Molecular Biology·Mirjam CzjzekYuval Shoham
Jan 31, 1997·The Journal of Biological Chemistry·S ArmandH Driguez
May 25, 2002·The Journal of Biological Chemistry·Maria HrmovaGeoffrey B Fincher

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