PMID: 8647088Apr 15, 1996

Stereochemical requirements of chitin synthase for ligand binding at the allosteric site for N-acetylglucosamine

European Journal of Biochemistry
M HorschD M Rast

Abstract

The substrate kinetics of chitin synthase (CS) were non-Michaelian, irrespective of the type of enzyme preparation studied (105-S chitosomal CS, and 16-S ex walls), even in the presence of saturating GlcNAc. An unexplained idiosyncrasy of this enzyme, which is likely to be responsible for this phenomenon, is evident from the striking non-linearity of product deposition with time at low substrate or low enzyme concentrations, particularly in the absence of GlcNac. The possibility can be excluded that this non-linearity is due to the formation of soluble by-products or intermediates in the form of chito-oligomers, as shown by HPLC/pulsed amperometric detection analysis. Additional evidence was sought for the tenet that CS is homotropically-heterotropically regulated, at least under steady-state reaction conditions. Substrate kinetic curves established from rate data for the linear reaction phase only were used for modelling. These could be reasonably well parameterised on the basis of the Monod mathematical model for co-operative ligand binding. Within a series of test compounds used to assess the stereochemical conditions of the allosteric site of CS for effector binding, N-acetylglucosaminono-1,5-lactone oxime excelled. Require...Continue Reading

References

Jan 1, 1990·Advances in Carbohydrate Chemistry and Biochemistry·G Legler
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C A Leal-MoralesS Bartnicki-Garcia
Jul 15, 1987·European Journal of Biochemistry·J Ricard, A Cornish-Bowden
Apr 1, 1966·Biochemistry·C A Porter, E G Jaworski
Jan 1, 1993·Journal of Enzyme Inhibition·M HorschD M Rast
Jul 1, 1993·Molecular & General Genetics : MGG·B CuberoE Cerdá-Olmedo
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX

Citations

Aug 29, 2003·Phytochemistry·D M RastG O Hollenstein
Jun 28, 2011·European Journal of Cell Biology·Hans Merzendorfer
Oct 26, 2016·Fungal Genetics and Biology : FG & B·Holjes Salgado-LugoJosé Ruiz-Herrera

Related Concepts

Acetylglucosamine
Allosteric Site
Chitin
Chitin Synthetase 2
Ligands
Mucor
Molecular Stereochemistry
Substrate Specificity

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