Stereocilia bundle stiffness: effects of neomycin sulphate, A23187 and concanavalin A

Hearing Research
M KösslI J Russell

Abstract

The effects that the aminoglycoside antibiotic neomycin sulphate, the ionophore A23187 and the lectin Concanavalin A (Con A) have on the steady state stiffness of the hair-cell stereocilia bundle have been studied using organotypic cultures of the early postnatal mouse cochlea. In normal saline, stereocilia bundle stiffness is increased 1.5-2.0 fold by neomycin sulphate, 1.3-1.7 fold by the ionophore A23187 and 3.0-5.0 fold by Con A. In low-calcium saline neither neomycin sulphate nor A23187 cause increases in stiffness, and the stiffness increases elicited by these two agents in normal saline are reversed on washout with low-calcium saline. In normal saline neomycin sulphate has two independent effects on hair cells; one effect is a reversible inhibition of transduction and the other effect is to cause an irreversible increase in bundle stiffness. Neither Con A nor the ionophore A23187 block transduction. No obvious changes in stereocilia bundle morphology are associated with the increases in stiffness caused by neomycin sulphate, A23187 and Con A. Succinylated Con A binds to stereocilia bundles but does not cause an increase in stiffness, suggesting that the stiffness increase caused by native Con A results from receptor cros...Continue Reading

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Citations

Jun 1, 1991·Hearing Research·G P Richardson, I J Russell
Apr 2, 1999·Trends in Pharmacological Sciences·J J Simpson, W E Davies
Jun 5, 2003·Hearing Research·Dennis M FreemanThomas F Weiss
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·S S Pae, J C Saunders
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Apr 7, 2005·Hearing Research·Jens MeyerAnthony W Gummer
Sep 20, 2005·Biophysical Journal·Dylan K Chan, A J Hudspeth
Aug 22, 1992·Proceedings. Biological Sciences·C J KrosG P Richardson

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