PMID: 9450914Feb 5, 1998Paper

Stereodependent inhibition of plasminogen activator inhibitor type 1 by phosphorothioate oligonucleotides: proof of sequence specificity in cell culture and in vivo rat experiments

Antisense & Nucleic Acid Drug Development
W J StecM Stasiak

Abstract

Hexadecadeoxyribonucleotides complementary to a fragment of human PAI-1 mRNA located upstream of the start codon and their phosphorothioate analogs were studied in cultured HUVECs as sequence-dependent inhibitors of PAI-1 expression. The activity of the random mixture of diastereomers of phosphorothioate hexadecanucleotide PS-16H has been compared with that of isosequential, stereoregular [All-Sp] and [All-Rp] isomers. The highest inhibitory effect on PAI-1 synthesis was observed with the [All-Sp] diastereomer. Stereorandom phosphorothioate oligonucleotide PS-16R complementary to the same region of rat PAI-1 mRNA, when injected into tail vein of rats, substantially decreased the level of PAI-1 in blood plasma.

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Citations

Sep 4, 1999·Nucleosides & Nucleotides·A KobylańskaW J Stec
Feb 6, 2003·Antisense & Nucleic Acid Drug Development·Maria MaszewskaMaria Koziołkiewicz
Mar 18, 2004·Oligonucleotides·Arthur M KriegWojciech Stec
May 11, 2000·Antisense & Nucleic Acid Drug Development·F Eckstein
Oct 30, 2014·Nucleic Acid Therapeutics·Fritz Eckstein
Oct 30, 2015·Future Medicinal Chemistry·Vivek K Sharma, Jonathan K Watts
Feb 3, 2004·Biopolymers·Shayantani Mukherjee, Dhananjay Bhattacharyya
Mar 6, 2004·Journal of Thrombosis and Haemostasis : JTH·K VerbekeP J Declerck
Sep 17, 2011·Chemistry & Biodiversity·Piotr Guga, Maria Koziołkiewicz
Mar 1, 2017·Nature Biotechnology·Anastasia Khvorova, Jonathan K Watts
Sep 5, 2017·Biomedical Chromatography : BMC·N M El ZaharMichael G Bartlett
Feb 2, 2019·Clinical and Translational Science·Wei Yin, Mark Rogge
Jun 27, 2020·Mass Spectrometry Reviews·James Michael SuttonMichael G Bartlett
Apr 2, 2020·Bioconjugate Chemistry·Daniel A GlazierWeiping Tang
Sep 28, 2000·Cancer Treatment Reviews·W PawlakC Szczylik

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