Stereological analysis of individual lung lobes during normal and aberrant mouse lung alveolarisation

Journal of Anatomy
Tuong-Van HoangRory E Morty

Abstract

The quantitative assessment of the lung architecture forms the foundation of many studies on lung development and lung diseases, where parameters such as alveoli number, alveolar size, and septal thickness are quantitatively influenced by developmental or pathological processes. Given the pressing need for robust data that describe the lung structure, there is currently much enthusiasm for the development and refinement of methodological approaches for the unbiased assessment of lung structure with improved precision. The advent of stereological methods highlights one such approach. However, design-based stereology is both expensive and time-demanding. The objective of this study was to examine whether 'limited' stereological analysis, such as the stereological analysis of a single mouse lung lobe, may serve as a surrogate for studies on whole, intact mouse lungs; both in healthy lungs and in diseased lungs, using an experimental animal model of bronchopulmonary dysplasia (BPD). This served the dual-function of exploring BPD pathobiology, asking whether there are regional (lobar) differences in the responses of developing mouse lungs to oxygen injury, by examining each mouse lung lobe separately in the BPD model. Hyperoxia expo...Continue Reading

References

Mar 1, 1990·Experimental Lung Research·T B ZeltnerP H Burri
Feb 16, 1967·The New England Journal of Medicine·W H NorthwayD Y Porter
Apr 7, 2000·Journal of Neuroscience Methods·J F LambertP J Quesenberry
Nov 1, 1960·Stain Technology·K C RICHARDSONE H FINKE
Sep 27, 2003·American Journal of Respiratory and Critical Care Medicine·Matthias OchsHans Jørgen G Gundersen
Feb 26, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·D M HydeH J G Gundersen
Sep 16, 2006·Journal of Applied Physiology·Ewald R WeibelMatthias Ochs
Feb 28, 2007·Toxicologic Pathology·Dallas M HydeCharles G Plopper
Dec 17, 2009·American Journal of Respiratory and Critical Care Medicine·Ali O YildirimHeinz Fehrenbach
Feb 5, 2010·American Journal of Respiratory and Critical Care Medicine·Connie C W HsiaUNKNOWN ATS/ERS Joint Task Force on Quantitative Assessment of Lung Structure
Aug 10, 2010·Current Topics in Developmental Biology·David WarburtonEdwin Jesudason
Mar 8, 2011·Journal of Microscopy·S A TschanzE R Weibel
Oct 3, 2012·Methods in Molecular Biology·Christian MühlfeldMatthias Ochs
Apr 16, 2013·Seminars in Perinatology·Bradley W BuczynskiMichael A O'Reilly
Apr 30, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Matthias Ochs, Christian Mühlfeld
May 28, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christian Mühlfeld, Matthias Ochs
Jan 1, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jan Philipp Schneider, Matthias Ochs
Nov 6, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Nathachit LimjunyawongWayne Mitzner
Jul 19, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christian MühlfeldMatthias Ochs
Sep 13, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Diogo M G SilvaRory E Morty
May 25, 2016·Molecular and Cellular Pediatrics·Claudio Nardiello, Rory E Morty
Jun 28, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Krithika LingappanBhagavatula Moorthy
Dec 6, 2016·Cell and Tissue Research·Claudio NardielloRory E Morty
Dec 17, 2016·Cell and Tissue Research·Ewald R Weibel
Jan 10, 2017·Disease Models & Mechanisms·Claudio NardielloRory E Morty
Feb 2, 2017·Cell and Tissue Research·Johannes C Schittny
Mar 21, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Agnieszka PozarskaRory E Morty
Oct 4, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·David E Surate SolaligueRory E Morty

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Citations

Oct 10, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ettore LignelliRory E Morty
Jan 28, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andrea PorzionatoMaurizio Muraca
Mar 31, 2021·American Journal of Respiratory Cell and Molecular Biology·Svenja V AppuhnChristian Mühlfeld

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