Stereological quantification of GAD-67-immunoreactive neurons and boutons in the hippocampus of middle-aged and old Fischer 344 x Brown Norway rats

The Journal of Comparative Neurology
Lei ShiJudy Karen Brunso-Bechtold

Abstract

The aging process in rodents is associated with learning and memory impairments that are correlated with changes in multiple neurotransmitter systems in the hippocampus. For example, the gamma-aminobutyric acid (GABA)ergic system is compromised in old compared with young rats (Shetty and Turner [1998] J. Comp. Neurol. 394:252-269; Vela et al. [2003] J. Neurochem. 85:368-377; Potier et al. [1992] Neuroscience 48:793-806; Potier et al. [1994] Brain Res. 661:181-188). The present study investigated the important issue of whether there is a decline of the GABAergic inhibitory system between middle and old age. Five middle-aged (15-17 months) and five old (25-29 months) Fischer 344 x Brown Norway male rats were perfused, and coronal sections through the dorsal hippocampus were immunoreacted with antibodies either to NeuN, a neuronal marker, or to the 67-kDa isoform of glutamic acid decarboxylase (GAD), the rate-limiting enzyme for GABA synthesis. Using the optical dissector technique, NeuN-immunoreactive (IR) cells, GAD-IR cells, and GAD-IR boutons were quantified stereologically in the dentate gyrus, CA3, and CA1. The resulting GAD-IR cell and GAD-IR bouton densities then were normalized to NeuN-IR cell density to exclude the possi...Continue Reading

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Citations

Jun 19, 2009·Journal of Neuropathology and Experimental Neurology·Crystal M DykstraJames W Gurd
Oct 22, 2008·Experimental Gerontology·Jana BurianovaJosef Syka
May 20, 2008·Experimental Gerontology·Ladislav OudaJosef Syka
Jun 12, 2013·The Journal of Comparative Neurology·Amy M SpiegelMichela Gallagher
Mar 21, 2013·Experimental Neurology·Youssef Hanna El-HayekLiang Zhang
Feb 1, 2011·Neurobiology of Aging·Emily M StanleyDavid D Mott
Nov 22, 2008·Biochimica Et Biophysica Acta·Shayna A WrightenLawrence P Reagan
May 14, 2008·International Journal of Radiation Oncology, Biology, Physics·Lei ShiJudy K Brunso-Bechtold
Oct 30, 2016·Neurobiology of Aging·Cindy Villanueva-CastilloEmilio J Galván
May 13, 2017·Aging Cell·Aleksandra Rozycka, Monika Liguz-Lecznar
Dec 26, 2017·Frontiers in Aging Neuroscience·Lara IanovThomas C Foster
May 1, 2009·Hippocampus·Susanne G Mueller, Michael W Weiner
Dec 25, 2009·The Journal of Comparative Neurology·Khushdev K ThindPaul S Buckmaster
Oct 19, 2006·Trends in Neurosciences·Iain A WilsonHeikki Tanila

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