PMID: 11318412Apr 25, 2001Paper

Stereoselective determination of ofloxacin and its metabolites in human urine by capillary electrophoresis using laser-induced fluorescence detection

Journal of Chromatography. B, Biomedical Sciences and Applications
C Horstkötter, G Blaschke

Abstract

A capillary electrophoresis method for the simultaneous separation and enantioseparation of the antibacterial drug ofloxacin and its metabolites desmethyl ofloxacin and ofloxacin N-oxide in human urine has been developed and validated. Enantioseparation was achieved by adding sulfobutyl beta-cyclodextrin to the running buffer. The detection of the analytes was performed by laser-induced fluorescence (LIF) detection using a HeCd-laser with an excitation wavelength of 325 nm. In comparison with conventional UV detection, LIF detection provides higher sensitivity and selectivity. The separation can be performed after direct injection of urine into the capillary without any sample preparation, because no matrix compounds interfere with the assay. Additionally, the high sensitivity of this method allows the quantification of the very low concentrations of enantiomers of both metabolites. The limit of quantification was 250 ng/ml for ofloxacin enantiomers and 100 ng/ml for each metabolites' enantiomers. This method was applied to the analysis of human urine samples collected from a volunteer after oral administration of 200 mg of (+/-)-ofloxacin to elucidate stereoselective differences in the formation and excretion of the metabolite...Continue Reading

Citations

Nov 5, 2008·Analytical and Bioanalytical Chemistry·Philipp Schulze, Detlev Belder
Jan 22, 2010·Analytical and Bioanalytical Chemistry·Manuel Lombardo-AgüíCarmen Cruces-Blanco
May 4, 2010·Analytical and Bioanalytical Chemistry·Leena Suntornsuk
Jul 26, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Georg Hempel
Mar 1, 2013·Journal of Basic and Clinical Pharmacy·Mahesh V Attimarad, Ahmed O Alnajjar
Jul 17, 2015·Biomedical Chromatography : BMC·Jing NieHong-Ping Xu
Jul 4, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ching-Ling ChengChen-Hsi Chou
Aug 5, 2009·Electrophoresis·Peter Mikus, Katarína Maráková
May 3, 2008·Luminescence : the Journal of Biological and Chemical Luminescence·Li ChenLinpei Jin
Oct 18, 2007·Electrophoresis·Yongxia FanWeidong Qin
Mar 11, 2016·Journal of Agricultural and Food Chemistry·Kuo HeShuo Wang
Jul 21, 2012·Journal of Chromatographic Science·Zhili FangWeiguang Zhang
May 17, 2017·Journal of Separation Science·Imran AliLeonid Asnin
Nov 24, 2004·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Li Ming DuMi Fu
May 1, 2009·Bioanalysis·Marilyn J Schneider
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Jan 9, 2019·Bioscience, Biotechnology, and Biochemistry·Xiuyuan ZhangZhihong Huang
May 24, 2006·Electrophoresis·Rukhsana JabeenJohn Petersen
Sep 1, 2005·Luminescence : the Journal of Biological and Chemical Luminescence·Xianhua ZhangHuichun Zhao
Mar 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana-Mădălina MăciucăValentina Uivarosi

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