Stereoselective modification of circular dichroism spectra of rat lung beta-adrenoceptor protein preparation by enantiomers of epinephrine

Popat N PatilP K Dutta


The presence of beta-adrenoceptor in a rat lung membrane preparation was confirmed by stereoselective competition of enantiomers of epinephrine with labeled iodocyanopindolol. The receptor-rich protein fraction, when combined with the pharmacologically active (-)-epinephrine, exhibited specific changes in the 205-220 nm region of circular dichroism spectra, indicating that the receptor helices may be perturbed. The (+)-epinephrine combined with the lung protein produced little or no change of the spectra. Bovine serum albumin, when combined with either enantiomer of epinephrine, produced nonstereoselective alterations of the spectra. Thus, the data provide important evidence for the higher intrinsic pharmacologic activity of the natural (-)-epinephrine over the unnatural (+)-enantiomer.


Nov 13, 2008·Molecular Pharmacology·Roland Seifert, Stefan Dove
Jan 4, 2008·Chirality·Popat N PatilJ Paul Hieble
Aug 29, 2017·Journal of Liquid Chromatography & Related Technologies·Douglas KirkpatrickMichael Trehy


Aug 1, 1976·European Journal of Pharmacology·R R RuffoloPopat N Patil
Dec 22, 1983·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J W Black, P Leff

Related Concepts

Molecular Stereochemistry
Tissue Membrane
Epinephrine Acetate
Beta-adrenergic receptor

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