PMID: 8263108Sep 22, 1993Paper

Stereospecific high-performance liquid chromatographic assay of ibuprofen: improved sensitivity and sample processing efficiency

Journal of Chromatography
C H LemkoR T Foster

Abstract

A high-performance liquid chromatographic (HPLC) assay suitable for the analysis of the enantiomers of the non-steroidal anti-inflammatory drug ibuprofen (IB) in plasma was developed. Following the addition of racemic fenoprofen as internal standard (I.S.), samples are acidified and extracted with a mixture of isooctane-isopropanol (95:5, v/v). After evaporation of the organic layer, the drug and I.S. are derivatized with S-(-)-1(1-naphthyl)ethylamine (S-NEA) after addition of ethyl chloroformate as the coupling reagent. Ethanolamine is added 3 min after the addition of S-NEA to react with the excessive ethyl chloroformate. The resultant diastereomers corresponding to IB and I.S. were chromatographed at ambient temperature on a 100 mm x 4.6 mm I.D. C18 reversed-phase column using acetonitrile-water-acetic acid-triethylamine (60:40:0.1:0.02) as the mobile phase pumped at a flow-rate of 1.2 ml/min. Detection of the fluorescent chromophore was at 280 and 320 nm for excitation and emission, respectively. The suitability of the assay for clinical pharmacokinetic studies of IB was determined by the analysis of plasma samples obtained from a healthy volunteer, following administration of a single 400-mg oral dose of racemic IB.

References

May 1, 1975·Journal of Pharmaceutical Sciences·G J Vangiessen, D G Kaiser
Sep 1, 1989·Journal of Pharmaceutical Sciences·F JamaliF M Pasutto
Dec 1, 1984·Analytical Chemistry·J B CrowtherJ D Henion

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Citations

Jun 12, 2008·European Journal of Drug Metabolism and Pharmacokinetics·Chaonan ZhengJing Li
Aug 5, 1994·Journal of Chromatography. B, Biomedical Applications·R T FosterF M Pasutto
Oct 29, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiao Wei TengNeal M Davies
Jan 6, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·B Vermeulen, J P Remon
Oct 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Henry FarrarMichael Gill
Jun 1, 1996·Journal of Veterinary Pharmacology and Therapeutics·J D RoderS Sangiah
Aug 4, 2001·Pharmaceutical Development and Technology·M K Ghorab, M C Adeyeye
Feb 23, 1999·Pharmaceutical Development and Technology·R K Janjikhel, C M Adeyeye
May 11, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Natalija NakovDobrin Svinarov
Dec 23, 1998·Biomedical Chromatography : BMC·R Bhushan, J Martens
Jul 3, 2013·Journal of Separation Science·Jolanta Flieger, Anna Czajkowska-Żelazko
Apr 11, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·N M Davies

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