Sterility testing of platelet concentrates prepared from deliberately infected blood donations

Transfusion
Harald MohrThomas H Müller

Abstract

In general the bacterial count in freshly donated blood is low and even lower in the corresponding platelet concentrates (PCs). By use of flow cytometry (FACS) for sterility testing, the reliability of early versus later sampling times was evaluated. Blood donations were spiked with various numbers of Staphylococcus epidermidis, Staphylococcus aureus, Bacillus cereus, and Klebsiella pneumoniae. The corresponding PCs were prepared by the buffy-coat method and stored at 22 degrees C. A 20-mL sample was collected from each PC directly after preparation and after 8 hours. Samples were stored at 35 degrees C. Sterility testing of both PCs and samples was by FACS analysis at different time points. All stored PCs were found positive by FACS analysis, with detection times ranging between 8 and 24 hours (K. pneumoniae, B. cereus), 8 and 91 hours (S. aureus), and 144 hours (S. epidermidis). In the samples incubated at 35 degrees C, bacteria were detected after 8 to 19 hours (K. pneumoniae, B. cereus), 8 to 67 hours (S. aureus), and 19 to 43 hours (S. epidermidis). Some of the samples did not contain bacteria. Detection times for slow-growing bacteria are significantly shortened when PC samples are incubated at 35 degrees C: the numbers o...Continue Reading

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Citations

Oct 22, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Michael SchmidtErhard Seifried
Oct 22, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Thomas H MüllerAxel W Seltsam
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Thomas H MüllerThomas Montag
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Oliver KaroThomas Montag
Jun 22, 2007·Transfusion·Carey GrecoSandra Ramírez-Arcos
Aug 27, 2013·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Bian GuoHuiLi Wuping

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